Characterisation of immunoglobulin-binding leucocytes in carp (Cyprinus carpio L.)
J C Koumans-van Diepen1, M H van de Lisdonk, A J Taverne-Thiele
1Department of Experimental Animal Morphology and Cell Biology, Agricultural University, Wageningen, The Netherlands.
Insights
Carp macrophages, particularly in the hindgut, exhibit immunoglobulin (Ig)-binding capabilities. This study identifies specific immune cells in carp capable of binding Ig, offering insights into fish immune responses.
Area of Science:
- Immunology
- Fish Biology
- Cellular Biology
Background:
- Immunoglobulin (Ig) binding is crucial for immune cell function.
- Understanding Ig-binding in fish immune cells is vital for aquaculture and conservation.
- Carp macrophages are key immune cells in fish.
Purpose of the Study:
- To investigate the immunoglobulin-binding capacity of carp immune cells.
- To characterize the specific cell types involved in Ig binding in carp.
- To explore the distribution of Ig-binding cells in different carp organs.
Main Methods:
- Immunofluorescence microscopy
- Immunogold labeling techniques
- Internalization assays
- Erythrocyte-antibody rosette assay
Main Results:
- Most hindgut macrophages demonstrated Ig-binding capacity after Ig internalization.
- A limited number of monocyte-like cells in the pronephros also showed Ig binding.
- Lymphoid cells in both hindgut and pronephros cell suspensions were found to bind Ig.
- Pronephros macrophages and neutrophilic granulocytes were Ig-negative.
- Rosette assays confirmed limited Ig-binding cell populations.
Conclusions:
- Carp macrophages, especially from the hindgut, possess significant Ig-binding potential.
- Specific monocyte-like and lymphoid cells in carp also exhibit Ig-binding properties.
- These findings contribute to the understanding of the innate and adaptive immune mechanisms in fish.
Abstract:
This study demonstrates the immunoglobulin(Ig)-binding capacity of Ig-positive carp macrophages employing immunofluorescence and immunogold methods. These methods allow for the characterisation of the Ig-binding cells. After internalisation of fluorescent- or gold-labelled Ig (30 min at room temperature), most macrophages from the hindgut were able to bind added purified carp Ig, which could be demonstrated clearly with a second fluorescent or gold label. In pronephros, an important haemopoietic organ in fish, a limited number of monocyte-like cells also showed Ig binding. Pronephros macrophages and neutrophilic granulocytes appeared to be Ig-negative. The use of goat anti-mouse Ig gold particles bound by carp anti-goat antibodies revealed that, in addition to hindgut macrophages and pronephric monocyte-like cells, some lymphoid cells in both hindgut and pronephros cell suspensions were also able to bind Ig. The classic erythrocyte-antibody rosette assay resulted in a limited number of small rosettes in cell suspensions from both organs.
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