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Characterization of neutrophils and T lymphocytes associated with the murine vaginal epithelium

D Nandi1, J P Allison

  • 1Department of Molecular and Cell Biology, University of California, Berkeley 94720.

Regional Immunology
|November 1, 1993
PubMed

Insights

This study identifies neutrophils and T cells as key leukocytes in the murine vaginal epithelium, revealing their stable presence throughout the estrous cycle and hormonal changes, crucial for mucosal immunity.

Area of Science:

  • Immunology
  • Reproductive Biology
  • Cell Biology

Background:

  • The murine vaginal epithelium is a stratified squamous epithelium that undergoes cyclical changes.
  • Understanding the immune cell populations within this tissue is crucial for comprehending mucosal immunity.

Purpose of the Study:

  • To characterize the types and distribution of leukocytes associated with the murine vaginal epithelium.
  • To investigate the influence of the estrous cycle and ovarian hormones on these leukocyte populations.

Main Methods:

  • Monoclonal antibodies targeting cell surface antigens were used.
  • Multiparameter flow cytometry was employed to analyze leukocyte populations.
  • Analysis was conducted across different phases of the estrous cycle and under hormonal influence.

Main Results:

  • Two main CD45+ leukocyte populations were identified: neutrophils (Mac-1+) and T cells (CD3+).
  • Alpha beta T cells, gamma delta T cells, and a novel Thy-1+CD3- population were associated with the epithelium.
  • Leukocyte composition remained stable during the estrous cycle and was unaffected by 17 beta-estradiol and progesterone.

Conclusions:

  • Neutrophils and specific T cell subsets are the primary leukocytes in the murine vaginal epithelium.
  • These immune cells are consistently present, independent of the estrous cycle and ovarian hormone levels.
  • The study provides a foundational characterization of leukocytes involved in murine vaginal mucosal immunity.

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