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In vitro Measurements of Tracheal Constriction Using Mice
10:20

In vitro Measurements of Tracheal Constriction Using Mice

Published on: June 25, 2012

Extracellular ATP regulation of feline tracheal submucosal gland secretion

S Shimura1, T Sasaki, M Nagaki

  • 1First Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.

The American Journal of Physiology
|August 1, 1994
PubMed

Insights

Adenosine triphosphate (ATP) stimulates electrolyte and mucus glycoprotein secretion in feline airway submucosal glands. Beta-receptor stimulation enhances this ATP-induced secretion via increased intracellular cyclic adenosine monophosphate (cAMP).

Area of Science:

  • Cellular Physiology
  • Respiratory System Biology
  • Glandular Secretion Mechanisms

Background:

  • Airway submucosal glands play a crucial role in mucus production and maintaining airway homeostasis.
  • Understanding the regulation of electrolyte and mucus secretion is vital for respiratory health research.
  • Adenosine triphosphate (ATP) and beta-adrenergic agonists are known modulators of airway secretions.

Purpose of the Study:

  • To investigate the effects of ATP and isoproterenol (ISO) on ion currents, intracellular calcium ([Ca2+]i), and mucus glycoprotein (MGP) secretion in feline airway submucosal glands.
  • To elucidate the role of beta-adrenergic receptor stimulation in modulating ATP-induced secretory responses.

Main Methods:

  • Patch-clamp electrophysiology was used to measure ion currents in isolated acinar cells.
  • Fura 2 fluorescence was employed to assess intracellular calcium ([Ca2+]i) levels.
  • Measurement of trichloroacetic acid-precipitable [3H]glycoconjugates quantified MGP secretion.

Main Results:

  • ATP evoked inward (Cl-) and outward (K+) currents, increased [Ca2+]i, and stimulated MGP secretion.
  • Isoproterenol (ISO) alone had no significant effect but augmented ATP-induced currents, [Ca2+]i, and MGP secretion.
  • The phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine mimicked ISO's augmentation, suggesting a role for cyclic adenosine monophosphate (cAMP).

Conclusions:

  • P2-receptor stimulation by ATP induces electrolyte and MGP secretion via intracellular calcium release.
  • Beta-receptor stimulation enhances ATP-induced secretion by increasing intracellular cAMP levels.
  • These findings highlight a synergistic mechanism involving purinergic and adrenergic signaling in regulating airway submucosal gland function.

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