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Immunohistochemical detection of intercellular adhesion molecule-1 (ICAM-1) and major histocompatibility complex
Y Tomita1, M Kimura, T Tanikawa
1Department of Urology, Niigata University School of Medicine, Japan.
Insights
Intercellular adhesion molecule-1 (ICAM-1) and major histocompatibility complex (MHC) antigens are expressed in seminoma, potentially aiding anti-tumor responses. However, expression was low in some cases despite significant lymphocyte infiltration.
Area of Science:
- Immunology
- Oncology
- Pathology
Background:
- Seminoma is a testicular germ cell tumor.
- Understanding the tumor microenvironment is crucial for developing effective therapies.
Purpose of the Study:
- To investigate the expression of ICAM-1 and MHC antigens in seminoma.
- To characterize tumor-infiltrating mononuclear cells (TIM) in seminoma.
Main Methods:
- Immunohistological examination of 10 seminoma specimens.
- Detection of ICAM-1, MHC class I and II antigens.
- Characterization of TIM, focusing on T cells expressing LFA-1.
Main Results:
- ICAM-1 and MHC class I antigens were variably expressed in seminoma, but not on normal spermatogenic cells.
- MHC class II antigens were not detected.
- TIM, mainly T cells expressing LFA-1, were present in all tumors.
- No correlation found between antigen expression and clinical/histopathological factors or lymphocyte infiltration.
Conclusions:
- ICAM-1 and MHC class I antigens are expressed in seminoma, suggesting a role in host anti-tumor immunity.
- Low antigen expression in some cases with high lymphocyte infiltration warrants further investigation.
Abstract:
Expression of intercellular adhesion molecule-1 (ICAM-1) and major histocompatibility complex (MHC) antigens, and characterization of tumor-infiltrating mononuclear cells (TIM) were examined immunohistologically in 10 specimens of seminoma. ICAM-1 and MHC antigens were not detected on normal spermatogenic cells. ICAM-1 and MHC class I antigens were variably expressed in 7 and 9 seminomas, respectively, whereas class II antigens were not detected. Although the degree of expression of ICAM-1 and MHC antigens was not correlated with any clinical or histopathological factors, neither of the antigens was detected on an anaplastic seminoma. Various numbers of TIM were detected in all of the seminoma, and comprised mainly T cells bearing the lymphocyte function-associated antigen (LFA)-1. No significant correlation was noticed between the degree of lymphocyte infiltration and ICAM-1 or MHC antigen expression. Although ICAM-1 and MHC class I antigens were expressed in seminoma, possibly facilitating an anti-tumor reaction of host, their expression remained low in several cases, despite marked lymphocyte infiltration within the tumor.
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