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Thy 1+ dendritic epidermal cells but not Langerhans cells express Ly 48

A A Gaspari1, B Ferbel, J G Frelinger

  • 1Department of Dermatology, University of Rochester School of Medicine and Dentistry, New York 14642.

The Journal of Investigative Dermatology
|March 1, 1993
PubMed

Insights

Leukosialin (Ly 48), a sialoprotein, is expressed by dendritic epidermal T cells (DETCs) within the epidermis. Further research is needed to understand the function of Ly 48 on DETCs and other T cells.

Area of Science:

  • Immunology
  • Dermatology
  • Cell Biology

Background:

  • Leukosialin (Ly 48) is a sialoprotein found on developing bone marrow cells.
  • Its expression on epidermal cells (ECs) is not well-defined.

Purpose of the Study:

  • To investigate Ly 48 expression on specific epidermal cell subsets.
  • To identify which epidermal cells express Ly 48.

Main Methods:

  • Flow cytometry using monoclonal antibodies against Ly 48.
  • Immunoblotting of epidermal cell lysates.
  • Cell population enrichment and depletion studies.

Main Results:

  • Approximately 2.0% of unfractionated ECs expressed Ly 48.
  • Immunoblots detected a 125 kD antigen reactive with Ly 48 antibodies.
  • Dendritic epidermal T cells (DETCs) were identified as the sole EC population expressing Ly 48.
  • Cultured DETC lines showed heterogeneous Ly 48 epitope expression.

Conclusions:

  • Dendritic epidermal T cells (DETCs) are the primary epidermal cell type expressing Leukosialin (Ly 48).
  • The functional significance of Ly 48 on DETCs and other T cells requires further investigation.

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