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Human immunodeficiency virus type 1 DNA synthesis, integration, and efficient viral replication in growth-arrested T

G Li1, M Simm, M J Potash

  • 1Molecular Virology Laboratory, St. Luke's/Roosevelt Hospital Center, New York, New York.

Journal of Virology
|July 1, 1993
PubMed

Insights

Human immunodeficiency virus type 1 (HIV-1) replicates in T cells regardless of cell division. This study demonstrates that HIV-1 expression and replication occur efficiently even when T cell division is inhibited.

Area of Science:

  • Virology
  • Immunology
  • Cell Biology

Background:

  • Human immunodeficiency virus type 1 (HIV-1) replicates in monocytes and macrophages but not resting T lymphocytes.
  • The role of T cell division in the HIV-1 replicative cycle remains unclear.

Purpose of the Study:

  • To investigate the contribution of cell division to HIV-1 replication in T cells.
  • To evaluate HIV-1 expression, proviral DNA integration, and progeny virus production in non-dividing T cells.

Main Methods:

  • C8166 T-lymphoid cells were treated with mitomycin or aphidicolin to block cell division.
  • HIV-1 expression, p24 antigen levels, HIV-1 specific antigens, viral DNA, and progeny virus activity were assessed.
  • Proviral DNA integration was analyzed using a polymerase chain reaction-based assay.

Main Results:

  • HIV-1 expression and replication were equivalent in both dividing and non-dividing C8166 cells.
  • Proviral DNA integration occurred in T cells irrespective of cell division.
  • Similar results were observed in growth-arrested Jurkat T-lymphoid cells.

Conclusions:

  • Cell division is not required for efficient HIV-1 expression in T cells.
  • Cellular DNA synthesis is not a prerequisite for HIV-1 replication in T lymphocytes.

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