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[The expression of ICAM-1 on head and neck squamous cell carcinoma cell lines]
T Yago1, M Tukuda, I Mochimatu
1Otorhinolaryngology, Yokohama City University School of Medicine.
Insights
Intercellular adhesion molecule-1 (ICAM-1) is expressed on head and neck squamous carcinoma cells. Interferon-gamma significantly increased ICAM-1 expression in all tested cell lines.
Area of Science:
- Immunology
- Cell Biology
- Oncology
Context:
- Intercellular adhesion molecule-1 (ICAM-1), a 90 KDa glycoprotein in the immunoglobulin superfamily, mediates immune cell interactions.
- ICAM-1 functions as a ligand for leukocyte integrin lymphocyte-function associated antigen-1 (LFA-1).
- Head and neck squamous cell carcinoma (HNSCC) is a significant global health concern, with cell adhesion molecules potentially influencing tumor progression.
Purpose:
- To investigate the expression levels of ICAM-1 on HNSCC cell lines.
- To determine the effect of interferon-gamma (IFN-gamma) on ICAM-1 expression in these cell lines.
Summary:
- Immunohistochemical analysis revealed varying baseline ICAM-1 expression across fifteen HNSCC cell lines, with seven exhibiting high expression (++).
- No correlation was found between the primary tumor site or pathological differentiation and ICAM-1 expression levels.
- Treatment with IFN-gamma consistently upregulated ICAM-1 expression to the highest category (++) in all tested HNSCC cell lines.
Impact:
- This study highlights IFN-gamma's potent ability to enhance ICAM-1 expression on HNSCC cells.
- Findings suggest a potential therapeutic strategy targeting ICAM-1/LFA-1 interactions in HNSCC treatment.
- Further research into modulating ICAM-1 expression could offer new avenues for HNSCC management.
Abstract:
Intercellular adhesion molecule-1 (ICAM-1) is one of the cell adhesion molecules. This molecule is a glycoprotein of about 90 KDa belonging to the immunoglobulin (Ig) superfamily and is widely expressed by hematopoietic and non-hematopoietic cells which play a role in the immune system. ICAM-1 is also a ligand or counter-receptor for the leukocyte integrin lymphocyte-function associated antigen-1 (LFA-1). We investigated the expression of ICAM-1 on the surfaces of cells from fifteen head and neck squamous carcinoma cell lines and the modulation of ICAM-1 expression by IFN-gamma, using an immunohistochemical stain. We categorized four types of stain degree. (-): < 10% of cells positive (+/-): 10-40% of cells positive (+): < 40-70% of cells positive (++): > 70% of cells positive Four cell lines showed (-) type. Three cell lines: (+/-). One cell line: (+). Seven cell lines: (++). The primary site of cell lines and the degree of ICAM-1 expression were not detectable. Connection between pathological differentiation and the degreed expression were not apparent, either. IFN-gamma up-regulated the degree of ICAM-1. All cell lines, when stimulated by IFN-gamma, showed (++) type.
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