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Updated: Aug 8, 2026

Isolation and Activation of Murine Lymphocytes
08:08

Isolation and Activation of Murine Lymphocytes

Published on: October 30, 2016

Lysozyme as a regulator of interleukin-2-activated lymphocyte proliferation

N Yabe1, K Komiya, T Takezono

  • 1Department of Hygiene, Dokkyo University School of Medicine, Tochigi, Japan.

In Vitro Cellular & Developmental Biology. Animal
|October 1, 1993
PubMed

Insights

Lysozyme

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Lysozyme is an enzyme with known antimicrobial properties.
  • Its role in modulating lymphocyte proliferation is not fully understood.

Purpose of the Study:

  • To investigate the dual effect of lysozyme on interleukin-2 (IL-2)-stimulated human peripheral blood lymphocyte proliferation.
  • To identify factors influencing lysozyme's augmentation or inhibition of lymphocyte response.

Main Methods:

  • Human peripheral blood lymphocytes were stimulated with varying concentrations of IL-2.
  • Lysozyme was added at different time points and concentrations.
  • Lymphocyte proliferation was assessed under conditions with and without serum and with varying lymphocyte activation states.

Main Results:

  • Lysozyme augmented IL-2-mediated proliferation at suboptimal IL-2 concentrations.
  • Lymphocyte activation with anti-CD3 antibody shifted augmentation to inhibition.
  • Eliminating MHC class II+ cells enhanced lysozyme's augmenting effect.
  • Early lysozyme addition (within 1 hour) promoted proliferation, while later addition was inhibitory.
  • Serum presence modulated lysozyme's effect, often retarding its action.

Conclusions:

  • Lysozyme exhibits context-dependent regulation of lymphocyte proliferation.
  • Its effects are influenced by IL-2 levels, lymphocyte activation, timing of addition, and serum presence.
  • Lysozyme may play a role in fine-tuning immune responses based on stimulus magnitude and cellular events.

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