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A review on Fc epsilon RI on human epidermal Langerhans cells
1Department of Internal Medicine, Sakura Hospital, Toho University, School of Medicine, Japan.
Insights
Human epidermal Langerhans cells (LC) express Fc epsilon RI. Monomeric IgE binding to LC was blocked only by anti-Fc epsilon RI antibodies, confirming Fc epsilon RI surface expression on these immune cells.
Area of Science:
- Immunology
- Dermatology
- Cell Biology
Background:
- Langerhans cells (LC) are key immune cells in the epidermis.
- The high-affinity receptor for IgE (Fc epsilon RI) plays a crucial role in allergic responses.
Purpose of the Study:
- To investigate the surface expression of Fc epsilon RI on human epidermal Langerhans cells.
- To determine if Fc epsilon RI is the primary receptor mediating IgE binding to LC.
Main Methods:
- Examined monomeric IgE binding to human epidermal LC.
- Utilized monoclonal antibodies (mAbs) against Fc epsilon RII/CD23 and Fc gamma RII/CD32.
- Used anti-Fc epsilon RI mAb 15-1 to block IgE binding.
- Tested the effect of lactose on IgE binding.
Main Results:
- The majority of epidermal LC demonstrated binding of monomeric IgE.
- IgE binding was not inhibited by anti-Fc epsilon RII/CD23 or anti-Fc gamma RII/CD32 mAbs, nor by lactose.
- Preincubation with anti-Fc epsilon RI mAb 15-1 completely abrogated IgE binding to LC.
Conclusions:
- Human epidermal LC express Fc epsilon RI molecules on their surface.
- Fc epsilon RI is the specific receptor responsible for IgE binding to epidermal LC.
Abstract:
In order to show the surface expression of Fc epsilon RI on human epidermal Langerhans cells (LC), we examined monomeric IgE binding to LC. We demonstrated that the majority of epidermal LC were able to bind monomeric IgE. IgE binding to LC could neither be prevented by preincubation of the tissue with monoclonal antibodies (mAb) against either Fc epsilon RII/CD23 or Fc gamma RII/CD32, nor by the addition of lactose, but could be entirely abrogated by preincubation with the anti-Fc epsilon RI mAb 15-1. These data show that epidermal LC express Fc epsilon RI molecules.