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Cultured human NK cells express the Ki-1/CD30 antigen
A Cambiaggi1, C Cantoni, S Marciano
1Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy.
Insights
Cultured human Natural Killer (NK) cells express the Ki-1/CD30 antigen, identified by the BER-H2 antibody. This finding may aid in classifying lymphoid cell cancers with unrearranged T-cell receptor genes.
Area of Science:
- Immunology
- Cell Biology
- Oncology
Background:
- The Ki-1/CD30 antigen is associated with Hodgkin's lymphoma.
- Natural Killer (NK) cells are crucial immune cells involved in tumor surveillance.
Purpose of the Study:
- To investigate the expression of the Ki-1/CD30 antigen on human NK cells.
- To explore the potential implications of CD30 expression on NK cells for lymphoid neoplasia classification.
Main Methods:
- In vitro culture of human polyclonal NK cell lines and clones.
- Immunophenotyping using the BER-H2 monoclonal antibody.
- Western blot analysis of cell lysates.
- Functional assays assessing NK cell cytotoxic activity.
Main Results:
- Cultured human NK cell lines and clones express the Ki-1/CD30 antigen (BER-H2+).
- BER-H2 antigen expression on NK cells is lower than on Hodgkin's lymphoma cell lines.
- Resting NK-enriched peripheral blood lymphocytes (PBLs) did not react with BER-H2 mAb.
- Western blot identified BER-H2-reactive molecules of approximately 110 kD and 100 kD in NK cells.
- The BER-H2 antibody did not inhibit NK cell activity or trigger redirected killing.
Conclusions:
- Cultured human NK cells express the Ki-1/CD30 antigen.
- This expression may be relevant for the lineage assignment of CD30+ lymphoid neoplasms with unrearranged T-cell receptor genes.
Abstract:
In this study we show that in vitro cultured human polyclonal NK cell lines and clones express the Ki-1/CD30 Hodgkin-associated antigen, identified by the BER-H2 monoclonal antibody. The percentage of BER-H2+ cells ranged from 19% to 67% in five polyclonal NK cell lines and was 31% and 20% in two NK clones. The intensity of BER-H2 mAb staining on cultured NK cells was remarkably lower than that found on the L540 Hodgkin's lymphoma cell line. Resting PBL populations that had been enriched for NK cells failed to react with the BER-H2 mAb. Western blot analysis performed on cell lysates from a polyclonal NK cell line and from the NK3.3 NK-like cell line revealed that BER-H2-reactive molecules consisted of two major bands of approximately 110 kD and 100 kD. Two bands displaying an identical electrophoretic mobility were also found in lysates of the L540 cell line. The BER-H2 mAb failed to inhibit the nonspecific activated killer activity of cultured NK cells against both K562 and MeWo tumour target cells. In addition, the BER-H2 mAb was unable to trigger the cytolytic activity of NK cells in a redirected killing assay. The observation that cultured human NK cells express the Ki-1/CD30 antigen may be of relevance for the possible lineage assignment of K11/CD30+ lymphoid cell neoplasia with unrearranged TCR genes.