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An Optimized Protocol for the Efficient Radiolabeling of Gold Nanoparticles by Using a 125I-labeled Azide Prosthetic Group
Published on: October 10, 2016
[Study on 125I-labeled human chorinic gonadotropin after purification through gel electrophoresis]
Insights
Radioiodination of human chorionic gonadotropin (HCG) with 125I results in activity loss during storage. Purification of 125I-HCG after storage significantly restores its binding capacities and biological activity.
Area of Science:
- Biochemistry
- Radiochemistry
- Immunology
Context:
- Radioiodination of human chorionic gonadotropin (HCG) using 125I sodium iodide and chloramine-T is a common method.
- Stored 125I-HCG exhibits significant degradation, leading to reduced immunological and biological activities over time.
Purpose:
- To investigate the impact of storage on the quality of radioiodinated HCG (125I-HCG).
- To evaluate the efficacy of polyacrylamide gel electrophoresis in purifying aged 125I-HCG and restoring its activity.
Summary:
- 125I-HCG stored for 30 days showed a decrease in immunological activity to 33.1% and biological activity to 44.0%.
- Purification of 30-day stored 125I-HCG using polyacrylamide gel electrophoresis increased binding capacities.
- Purified 125I-HCG demonstrated combination rates of 90.5% with antibodies and 95.3% with receptors, comparable to freshly labeled HCG.
Impact:
- Purification of stored 125I-HCG is crucial for maintaining assay reliability and experimental accuracy.
- This method enhances the usability of radioiodinated HCG for diagnostic and research applications.
- Suggests a practical approach to overcome the limitations of radiolabeled hormone stability.
Abstract:
Radioiodination of HCG with 125I Na using chloramine-T is widely employed. However, 125I-HCG damages in storage and thus results in decrease of immunological and biological activities. When 125I-HCG was in store as long as 30 days, the immunological activity and biological activity decreased from 100% to 33.1% and 44.0%, respectively. We used polyacrylamide gel electrophoresis to purify 125I-HCG which had been kept in store for 30 days since radiolabeling, and we found the binding capacities of purified 125I-HCG increased. The combination rates of purified 125I-HCG with antibody and receptors accounted for 90.5% and 95.3% of those of 125I-HCG 1 day after radiolabeling. These results suggest that 125I-HCG which has been stored for a period since radiolabeling should be purified before use.
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