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Cellular fibronectin on intraocular lenses explanted from patients

S Saika1, S Kobata, O Yamanaka

  • 1Department of Ophthalmology, Wakayama Medical College, Japan.

Graefe'S Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Fur Klinische Und Experimentelle Ophthalmologie
|December 1, 1993
PubMed

Insights

Fibronectin (FN) deposits on explanted intraocular lenses (IOLs) originate from adhered cells, likely macrophages. Immunohistochemistry confirmed FN presence in cellular and proteinaceous deposits on these IOLs.

Area of Science:

  • Ophthalmology
  • Biomaterials Science
  • Cell Biology

Background:

  • Intraocular lenses (IOLs) are implanted during cataract surgery.
  • Protein deposition on IOLs can affect visual outcomes.
  • The origin of fibronectin (FN) on explanted IOLs requires clarification.

Purpose of the Study:

  • To investigate the cellular origin of fibronectin (FN) deposits on explanted intraocular lenses (IOLs).

Main Methods:

  • Immunohistochemical analysis was performed on explanted posterior and anterior chamber IOLs.
  • Antibodies against cellular fibronectin were used to detect its presence.
  • Cellular and proteinaceous deposits on the IOLs were examined.

Main Results:

  • Both cellular deposits, presumed to be macrophages, and fibrous/membrane-like proteinaceous deposits on IOLs showed immunoreactivity to anti-FN antibodies.
  • The findings indicate that fibronectin is present in both cellular and extracellular matrix components on the IOL surface.
  • The proteinaceous deposits are likely products of cells adhering to the IOLs.

Conclusions:

  • Fibronectin found on explanted IOLs originates from adhered cells, primarily macrophages.
  • Understanding FN deposition is crucial for improving IOL biocompatibility and long-term performance.
  • Further research can explore strategies to mitigate unwanted protein adhesion on IOLs.

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