Related Experiment Video
Updated: Aug 8, 2026
Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
Published on: April 8, 2016
Ki-1-positive anaplastic large cell lymphoma with different phenotypes in skin nodules and lymph nodes
1Clinical Laboratory Section, Hospital, National Institute of Radiological Sciences, Chiba, Japan.
Insights
This study reports a case of Ki-1-positive anaplastic large cell lymphoma (ALCL) presenting with distinct cellular phenotypes in lymph nodes and skin. The findings suggest a potential co-occurrence with lymphomatoid papulosis or metastasis.
Area of Science:
- Dermatology
- Oncology
- Pathology
Background:
- Anaplastic large cell lymphoma (ALCL) is a distinct type of non-Hodgkin lymphoma.
- Cutaneous involvement in ALCL can present with varied clinical and immunophenotypic features.
Abstract:
A 54-year-old man had lymph node swelling and skin nodules. Neoplastic cells with pleomorphic nuclei, prominent eosinophilic nucleoli and abundant cytoplasm were present in the lymph node sinuses and around the dermal appendages. Neoplastic cells in the lymph nodes expressed Ber-H2 (CD30)+/KP1 (CD68)+/MAC387-/LN-5+. Those in the skin nodules expressed Ber-H2+/KP1-/MAC387+/LN-5-. These findings suggest that he had Ki-1-positive anaplastic large cell lymphoma (ALCL) with different phenotypes in lymph nodes and skin nodules. The discrepancy among KP1, MAC387 and LN-5 reactivities may represent the concurrent occurrence of ALCL and lymphomatoid papulosis. However, the possibility cannot be ruled out that ALCL developed first in the lymph nodes and then metastasized to the skin.
