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Published on: November 27, 2014
Analysis of the gamma/delta T cell restricted antigen WC1
Insights
Researchers identified two forms of the WC1 molecule using immunoprecipitation. These WC1 isoforms may help identify specific subpopulations of gamma/delta T cells.
Area of Science:
- Immunology
- Cell Biology
Background:
- WC1 antibodies are crucial for identifying gamma/delta T cells.
- Previous studies have suggested heterogeneity within WC1+ T cell populations.
Purpose of the Study:
- To investigate the molecular forms of WC1 recognized by a panel of monoclonal antibodies (mAbs).
- To determine if different WC1 isoforms correlate with distinct T cell subpopulations.
Main Methods:
- Immunoprecipitation and SDS-PAGE to analyze molecular weights.
- Flow cytometry for expression analysis and subclustering.
- Immunohistology for tissue distribution assessment.
Main Results:
- Two molecular forms of WC1, 205 kDa and 215 kDa, were identified.
- The expression of these WC1 forms correlated with flow cytometry-defined subclusters.
- No tissue-specific distribution of WC1 was observed via immunohistology.
Conclusions:
- The study provides evidence for at least two WC1 isoforms.
- These WC1 isoforms may serve as markers for identifying CD2- CD4- CD8- gamma/delta T cell subpopulations.
Abstract:
The nine antibodies (mAbs) which clustered with the standard WC1 mAb CC15 in temporary cluster TC22 were analysed by immunoprecipitation, flow cytometry and immunohistology. Two forms of the molecule, with molecular weights of 205 and 215 kDa, were identified by immunoprecipitation and SDS-PAGE, the expression of which correlated with subclusters generated by flow cytometric analysis. Immunohistological data, however, did not reveal any tissue specific distribution. We propose the existence of at least two isoforms of WC1 which may prove to identify subpopulations of CD2- CD4- CD8- gamma/delta T cells.
