Related Experiment Video

Updated: Aug 8, 2026

Flash Freezing and Cryosectioning E12.5 Mouse Brain
28:32

Flash Freezing and Cryosectioning E12.5 Mouse Brain

Published on: May 28, 2007

A simple method of rapid freezing adequately preserves brain tissue for immunocytochemistry, light and electron

D Nochlin1, A P Mackenzie, E M Bryant

  • 1Department of Pathology, University of Washington School of Medicine, Seattle 89195-0001.

Acta Neuropathologica
|January 1, 1993
PubMed

Insights

This study presents a straightforward method for freezing Alzheimer's disease brain tissue. This technique allows for the preservation of specific brain regions for various detailed analyses.

Area of Science:

  • Neuroscience
  • Pathology
  • Biotechnology

Background:

  • Alzheimer's disease research requires well-preserved brain tissue.
  • Existing cryopreservation methods may be complex or yield suboptimal results.

Purpose of the Study:

  • To develop a simple and reproducible cryopreservation technique for Alzheimer's disease brain tissue.
  • To ensure the integrity of brain tissue for diverse downstream analyses.

Main Methods:

  • Utilizing fresh brain slices (1 cm thick) obtained within 6 hours postmortem.
  • Employing a sandwich method with aluminum sheets and dry ice for rapid freezing.
  • Storing frozen tissue at -85°C.

Main Results:

  • Successful cryopreservation of brain tissue was achieved.
  • The method is simple, reproducible, and preserves tissue integrity.
  • Specific anatomic areas remain retrievable for analysis.

Conclusions:

  • The described method offers a reliable approach for cryopreserving Alzheimer's disease brain tissue.
  • Preserved tissue is suitable for a wide range of studies, including microscopy and neurochemistry.
  • This technique facilitates advanced research into Alzheimer's disease pathology.

More Related Videos

Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy11:55

Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy

Published on: April 3, 2017

Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy09:03

Rapid Freezing using Sandwich Freezing Device for Good Ultrastructural Preservation of Biological Specimens in Electron Microscopy

Published on: July 19, 2021

Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...