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VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma
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VDJ-Seq: Deep Sequencing Analysis of Rearranged Immunoglobulin Heavy Chain Gene to Reveal Clonal Evolution Patterns of B Cell Lymphoma

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Multiple breakpoints within the BCL-1 locus in B-cell lymphoma: rearrangements of the cyclin D1 gene

C J de Boer1, S Loyson, P M Kluin

  • 1Department of Pathology, University of Leiden, The Netherlands.

Cancer Research
|September 15, 1993
PubMed

Insights

This study investigates BCL-1 rearrangements in centrocytic lymphoma (CC) and intermediately differentiated lymphocytic lymphoma (IDL). Southern blot analysis revealed rearrangements in 50% of CC/IDL cases, confirming their association with the t(11;14) translocation.

Area of Science:

  • Hematology
  • Oncology
  • Cytogenetics

Background:

  • Centrocytic lymphoma (CC) and intermediately differentiated lymphocytic lymphoma (IDL) are B-cell non-Hodgkin's lymphomas.
  • These lymphomas are associated with the t(11;14)(q13;q32) chromosomal translocation.
  • Previous research suggested a link between this translocation and BCL-1 gene rearrangements.

Purpose of the Study:

  • To evaluate the association between BCL-1 gene rearrangements and CC/IDL.
  • To determine the utility of Southern blot analysis in identifying these lymphomas.

Main Methods:

  • Southern blot analysis was performed on 20 CC/IDL cases, 22 similar lymphomas, 11 chronic B-cell leukemias, and 2 myelomas.
  • Various probes were used to cover the BCL-1 locus.
  • Comigration analysis confirmed the t(11;14)(q13;q32) chromosomal rearrangement.

Main Results:

  • Rearrangements within the BCL-1 locus were detected in 50% (10 of 20) of CC/IDL cases.
  • Breakpoints varied, with some in the major translocation cluster (MTC) and others in different regions, including near the PRAD1/CCND1 gene.
  • All confirmed cases exhibited the t(11;14)(q13;q32) translocation.

Conclusions:

  • Southern blot analysis is a valuable tool for identifying CC/IDL.
  • Multiple breakpoints exist across a large region of the BCL-1 locus, necessitating the use of multiple probes for comprehensive detection.
  • The study reinforces the association between BCL-1 rearrangements and the t(11;14) translocation in CC/IDL.

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