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Characterization of a full-length cDNA encoding a bovine four tandem-repeat ubiquitin

F Wempe1, K H Scheit

  • 1Max-Planck-Institut für biophysikalische Chemie, Department of Molecular Biology, Göttingen, Germany.

Biochimica Et Biophysica Acta
|February 20, 1993
PubMed

Insights

Researchers identified two bovine ubiquitin clones from a seminal vesicle cDNA library. One clone showed a unique serine-to-phenylalanine substitution, potentially due to allelic polymorphism in ubiquitin gene expression.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Ubiquitin is a crucial protein involved in various cellular processes, including protein degradation and gene regulation.
  • The bovine seminal vesicle is a source of genetic material for studying protein expression.

Purpose of the Study:

  • To screen a bovine seminal vesicle cDNA library for ubiquitin-encoding clones.
  • To analyze the sequence and identify any variations in the ubiquitin repeats.

Main Methods:

  • Screening of a lambda gt11 cDNA library using monospecific anti-ubiquitin immunoglobulin Gs (IgGs).
  • DNA sequencing and amino acid sequence analysis of isolated clones (pUF4AA and pUD4AA).

Main Results:

  • Two independent clones, pUF4AA and pUD4AA, were isolated.
  • pUF4AA contained a polyubiquitin coding region with four tandem repeats, featuring a serine to phenylalanine substitution at position 133 in the second repeat.
  • pUD4AA exhibited the typical serine residue at position 133.

Conclusions:

  • The identified serine-phenylalanine substitution in bovine ubiquitin may represent an allelic polymorphism.
  • Further investigation is warranted to understand the functional implications of this variation in ubiquitin gene expression.

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