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Induction of CREB activity via the surface Ig receptor of B cells

H Xie1, T C Chiles, T L Rothstein

  • 1Department of Microbiology, Boston University Medical Center, MA 02118.

Journal of Immunology (Baltimore, Md. : 1950)
|July 15, 1993
PubMed

Insights

This study shows that CREB (cAMP response element-binding protein) is present in B cells and regulates gene expression. Surface Ig signaling, alone or with cAMP, controls CREB activity, indicating pathway cross-talk.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Signaling

Background:

  • Cyclic AMP (cAMP)-derived signals play a role in B cell activation.
  • The function and regulation of CREB (cAMP response element-binding protein) in B cells remain largely unelucidated.

Purpose of the Study:

  • To investigate the role and regulation of CREB in B cell activation.
  • To elucidate the impact of cAMP-derived signals on B cell function.

Main Methods:

  • Electrophoretic mobility shift assay (EMSA) to detect CRE-binding activity in B cell nuclear extracts.
  • Transient transfection of a B lymphoma cell line (BAL-17) with a CRE-dependent reporter construct.
  • Assessment of reporter gene activity following stimulation with anti-Ig and forskolin.

Main Results:

  • Constitutive CRE-binding activity, identified as CREB, was detected in primary B cells.
  • Surface Ig receptor cross-linking induced reporter gene activity in transfected B cells.
  • Forskolin alone did not stimulate reporter activity, but acted synergistically with anti-Ig, suggesting cross-talk between signaling pathways at the CREB level.

Conclusions:

  • CREB is present and functional in B cells.
  • CRE-dependent gene expression is regulated by surface Ig signaling, with potential synergy from cAMP-mediated pathways.
  • Evidence suggests cross-talk between intracellular signaling pathways impacting CREB activity in B cells.

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