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Updated: Aug 13, 2026

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
Published on: May 1, 2015
[The morphofunctional instability of the lymphocyte membranes in patients with chronic kidney failure]
Insights
Chronic renal failure (CRF) damages T-lymphocyte membranes, impairing E-receptor activity, lipid bilayer mobility, and transport. This morphofunctional damage contributes to lymphocytopenia and secondary immunodeficiency in CRF patients.
Area of Science:
- Immunology
- Nephrology
- Cell Biology
Abstract:
A rosette test, fluorescent probing (1-aniline-naphthaline-8-sulfonate and pyrene) and 14C-uridine inclusion into immuno-competent cells were used to investigate T-lymphocyte membrane E-receptor activity, mobility of the surface and deep parts of lymphocytic membrane lipid bilayer, and membrane transport, respectively. A total of 16 patients with conservatively curable and 31 patients with a terminal stage of chronic renal failure (CRF) were examined. The lymphocytic membranes were found unstable: E-receptor activity of RFC changed, microviscosity of the surface and deep lipid bilayer diminished, the transport function got disturbed. These phenomena significantly progress with deterioration of renal function. It is evident that morphofunctional damage to lymphocytic membranes may be a reason of lymphocytopenia and secondary immunodeficiency in CRF patients.
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