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Updated: Aug 8, 2026

Stereological and Flow Cytometry Characterization of Leukocyte Subpopulations in Models of Transient or Permanent Cerebral Ischemia
12:42

Stereological and Flow Cytometry Characterization of Leukocyte Subpopulations in Models of Transient or Permanent Cerebral Ischemia

Published on: December 28, 2014

Leukocyte infiltration in acute hemispheric ischemic stroke

P Y Wang1, C H Kao, M Y Mui

  • 1Department of Internal Medicine, Taichung Veterans General Hospital, Taiwan, ROC.

Stroke
|February 1, 1993
PubMed

Insights

Leukocyte infiltration in acute hemispheric ischemic stroke is a dynamic process. Using technetium-99m hexamethylpropyleneamine oxime (99mTc HMPAO)-labeled leukocyte brain SPECT, researchers found infiltration persists for at least 5 weeks before declining.

Area of Science:

  • Neurology
  • Nuclear Medicine
  • Immunology

Background:

  • Cerebral ischemia, particularly hemispheric ischemic stroke, involves complex inflammatory processes.
  • Understanding leukocyte dynamics is crucial for managing stroke outcomes.

Purpose of the Study:

  • To investigate the temporal dynamics of leukocyte infiltration in human cerebral ischemia.
  • To evaluate the utility of technetium-99m hexamethylpropyleneamine oxime (99mTc HMPAO)-labeled leukocyte brain single-photon emission computed tomography (SPECT) for this purpose.

Main Methods:

  • Twenty-two patients with hemispheric ischemic stroke (19 acute, 3 chronic) underwent 99mTc HMPAO brain SPECT for perfusion and leukocyte infiltration assessment.
  • Follow-up SPECT scans were performed at 1-3 week intervals for acute stroke patients.

Main Results:

  • In chronic stroke, no leukocyte infiltration was observed in perfusion defect areas.
  • In acute stroke, leukocyte infiltration was detected in perfusion defects during the acute phase.
  • This infiltration persisted for a minimum of 5 weeks post-onset before showing a decline.

Conclusions:

  • A novel method utilizing 99mTc HMPAO-labeled leukocyte brain SPECT enables the study and monitoring of leukocyte infiltration in acute cerebral ischemia.
  • Leukocyte infiltration in acute hemispheric ischemic stroke is a dynamic process with a prolonged duration of at least 5 weeks.
Abstract

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