Related Experiment Video
Updated: Aug 8, 2026
A Cell Free Assay System Estimating the Neutralizing Capacity of GM-CSF Antibody using Recombinant Soluble GM-CSF Receptor
Published on: June 27, 2011
Functional expression of interleukin 2 receptor in a human factor-dependent megakaryoblastic leukemia cell line:
Y Kanakura1, H Sugahara, H Mitsui
1Second Department of Internal Medicine, Osaka University Medical School, Japan.
Insights
Interleukin 2 receptors (IL-2R) are functionally expressed on acute myeloblastic leukemia cells. This suggests Interleukin 2 (IL-2) may impact human myelopoiesis and leukemia cell proliferation.
Area of Science:
- Immunology
- Hematology
- Molecular Biology
Background:
- Interleukin 2 (IL-2) regulates lymphocyte proliferation via the IL-2 receptor (IL-2R).
- The expression and function of IL-2R in acute myeloblastic leukemia (AML) cells remain largely uncharacterized.
- The M07E cell line, dependent on granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-3, or stem cell factor, serves as a model for myeloid leukemia.
Purpose of the Study:
- To investigate the expression and function of IL-2R on M07E myeloid leukemia cells.
- To determine if IL-2 influences the proliferation of M07E cells.
- To explore the potential role of IL-2 in human myelopoiesis and AML.
Main Methods:
- Radioligand binding assays using 125I-IL-2.
- Affinity cross-linking analyses.
- Immunoblotting with a monoclonal antibody (Mik-beta 1) against IL-2R beta.
- Quantitative analysis of IL-2R beta mRNA and protein levels.
- Competitive binding assays to assess interactions between IL-2 and GM-CSF.
Main Results:
- IL-2 stimulated proliferation of M07E cells in a dose-dependent manner.
- IL-2 augmented GM-CSF- and stem cell factor-induced proliferation of M07E cells.
- IL-2R beta was detected on M07E cells via binding assays, cross-linking, and antibody detection.
- GM-CSF treatment upregulated IL-2R beta mRNA and protein levels.
- GM-CSF competed with IL-2 for binding to IL-2R on M07E cells, indicating a potential functional interaction.
Conclusions:
- IL-2R is functionally expressed on M07E myeloid leukemia cells.
- IL-2 can directly stimulate M07E cell proliferation and enhance responses to other growth factors.
- These findings suggest a potential role for IL-2 in human myelopoiesis and the pathogenesis of AML.
Abstract:
Human interleukin 2 (IL-2) is a member of the class of crucial regulators of lymphocyte proliferation. The action of IL-2 is known to be mediated through binding to a specific IL-2 receptor (IL-2R) which comprises at least two distinct proteins: IL-2R alpha (p55) and IL-2R beta (p70-75). However, the expression and function of IL-2R are largely unknown in acute myeloblastic leukemia cells. In a human granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-3, or stem cell factor-dependent myeloid leukemia cell line (M07E), IL-2 was found to stimulate proliferation in a dose-dependent manner and to augment GM-CSF- and stem cell factor-induced proliferation of M07E cells. The expression of IL-2R beta on M07E cells was detectable with 125I-IL-2 binding and affinity cross-linking analyses and with a monoclonal antibody against IL-2R beta, Mik-beta 1. Although the expression of IL-2R beta was not down-regulated but somewhat up-regulated by treatment with GM-CSF in both mRNA and protein levels, GM-CSF was found to compete (75%) with radiolabeled IL-2 for binding to IL-2R on M07E cells, whereas no competition of GM-CSF binding was observed with IL-2 even at a 400-fold molar excess. These results suggest that IL-2R may be functionally expressed in some cases of acute myeloblastic leukemia cells and raise the possibility that IL-2 may have some effects on human myelopoiesis.
Related Concept Videos
01:15Differentiation of Common Myeloid Progenitor Cells
01:01Regulation of Hematopoietic Stem Cells