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Enhancement of lymphocyte function and interleukin 1 beta transcription by recombinant bovine interleukin 1 beta

J R Stabel1, M E Kehrli, J P Goff

  • 1USDA, Agricultural Research Service, Ames, IA 50010.

American Journal of Veterinary Research
|January 1, 1993
PubMed

Insights

Recombinant bovine interleukin 1 beta (rBoIL-1 beta) administration in cows enhanced immune cell function, increasing lymphocyte activity and IL-1 beta mRNA. This cytokine therapy may help alleviate immunosuppression in cattle.

Area of Science:

  • Veterinary Immunology
  • Cytokine Biology
  • Bovine Health

Background:

  • Interleukin-1 beta (IL-1 beta) is a key cytokine involved in immune responses.
  • Understanding the immunomodulatory effects of IL-1 beta in cattle is crucial for developing therapeutic strategies.
  • Recombinant bovine IL-1 beta (rBoIL-1 beta) offers a potential tool for immune modulation in livestock.

Purpose of the Study:

  • To evaluate the immunomodulatory effects of rBoIL-1 beta in nonpregnant, nonlactating Jersey cows.
  • To assess the impact of rBoIL-1 beta on immune cell function, including leukocytosis and lymphocyte activity.
  • To investigate the potential for IL-1 beta to upregulate its own production in bovine immune cells.

Main Methods:

  • Six Jersey cows received rBoIL-1 beta (166 ng/kg body weight) every 8 hours for 96 hours.
  • Leukocyte counts, lymphocyte blastogenesis, and mitochondrial activity were measured.
  • mRNA transcription of IL-1 beta in stimulated blood mononuclear cells was analyzed.

Main Results:

  • Persistent leukocytosis was observed within 3 hours of treatment, peaking at 24 hours.
  • rBoIL-1 beta stimulated lymphocyte blastogenesis and mitochondrial activity in resting and stimulated cell cultures.
  • IL-1 beta mRNA transcription increased significantly within 24 hours, indicating self-upregulation.

Conclusions:

  • Administration of rBoIL-1 beta enhances bovine immune cell function, particularly lymphocyte activity.
  • IL-1 beta may upregulate its own production in mononuclear cells, suggesting a positive feedback loop.
  • rBoIL-1 beta shows potential as a therapeutic agent for alleviating immunosuppression in cattle.

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