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Updated: Aug 12, 2026
Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
Published on: April 8, 2016
Immunophenotypic and genotypic analysis in cutaneous lymphoid hyperplasias
E Hammer1, O Sangueza, P Suwanjindar
1Department of Pathology, Oregon Health Sciences University 97201.
Insights
Immunophenotyping and Southern blot analysis can help diagnose lymphoma in cutaneous lymphoid hyperplasia (CLH) patients. Southern blot analysis may predict which CLH patients will develop lymphoma, aiding in treatment decisions.
Area of Science:
- Dermatopathology
- Oncology
- Immunology
Background:
- The diagnostic and prognostic utility of immunophenotyping and Southern blot analysis in cutaneous lymphoid hyperplasia (CLH) remains debated.
- Evaluating these techniques is crucial for accurate patient management.
Purpose of the Study:
- To assess the diagnostic and prognostic value of immunophenotyping and Southern blot analysis in patients diagnosed with CLH.
- To determine if these methods improve the evaluation of CLH cases.
Main Methods:
- Immunophenotyping was conducted on 26 skin biopsy specimens from patients with CLH.
- Southern blot analysis for immunoglobulin gene rearrangements was performed on 13 of these cases.
Main Results:
- Immunophenotyping identified 2 of 26 patients with monoclonal lymphoma; the rest showed polyclonal CLH.
- Southern blot analysis revealed clonal immunoglobulin gene rearrangements in 2 of 11 patients with polyclonal CLH, both of whom later developed lymphoma.
- Additional patients with polyclonal CLH also developed lymphoma, with varying Southern blot findings.
Conclusions:
- Immunophenotyping can detect lymphoma in cases with ambiguous histologic features.
- Southern blot analysis may identify patients with polyclonal CLH at risk for developing lymphoma, potentially guiding therapy.
Background:
The clinical utility of immunophenotyping and Southern blot analysis in the evaluation of patients with cutaneous lymphoid hyperplasia (CLH) is controversial.
Objective:
Our purpose was to determine whether adjunctive immunophenotyping and Southern blot analysis are of diagnostic and prognostic value in patients with CLH.
Methods:
Immunophenotyping was performed on skin biopsy specimens from 26 patients with a routine histologic diagnosis of CLH. Southern blot analysis for immunoglobulin (Ig) gene rearrangements was done on 13 of 26 cases.
Results:
Twenty-four of 26 patients had polyclonal CLH on immunophenotyping: 2 of 26 had monoclonal lymphoma. Two of 11 patients with polyclonal CLH studied by Southern blot analysis had clonal Ig gene rearrangements. In both, lymphoma developed within 1 to 6 years; comparison of CLH and malignant lymphoma demonstrated overlapping and different clonal bands. Two additional patients with polyclonal CLH developed lymphoma. No clonal gene rearrangements were detected in the CLH or lymphoma from one; the other was not studied.
Conclusion:
Immunophenotyping will identify some patients with lymphoma with nondiagnostic histologic features. Southern blot analysis will predict some patients with polyclonal CLH in whom malignant lymphoma will develop and who may benefit from definitive therapy.
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