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Signal transduction pathways mediating mucin secretion from intestinal goblet cells

T E Phillips1, J Wilson

  • 1Division of Biological Sciences, University of Missouri, Columbia 65211.

Digestive Diseases and Sciences
|June 1, 1993
PubMed

Insights

Cholinergic stimulation enhances mucin secretion in HT29-18N2 goblet cells via a non-compound exocytotic pathway, independent of protein kinase C. This reveals distinct signaling mechanisms for goblet cell responses.

Area of Science:

  • Cell Biology
  • Gastroenterology
  • Signal Transduction

Background:

  • Goblet cells are crucial for intestinal mucus secretion.
  • Understanding goblet cell secretion pathways is vital for gut health research.

Purpose of the Study:

  • To investigate the signaling pathways regulating mucin secretion in HT29-18N2 goblet cells.
  • To differentiate between compound and non-compound exocytotic pathways in goblet cell secretion.

Main Methods:

  • Utilized mucin-specific immunoassays.
  • Employed whole-mount immunocytochemistry.
  • Performed morphometric quantification of intracellular mucous granule stores.
  • Assessed the role of protein kinase C (PKC) and calcium ionophore A23187.

Main Results:

  • Cholinergic stimulation significantly increased mucin secretion.
  • Secretory response was mediated by a non-compound exocytotic pathway, not involving PKC.
  • Calcium ionophore A23187 also induced secretion via a non-compound exocytotic route.
  • Phorbol 12-myristate 13-acetate (PKC activator) stimulated secretion through a compound exocytotic pathway.

Conclusions:

  • Cholinergic stimulation triggers mucin secretion in goblet cells through a distinct non-compound exocytotic pathway.
  • Goblet cell secretion involves multiple signaling cascades, including PKC-dependent and independent routes.
  • These findings offer insights into the complex regulation of intestinal mucus production.

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