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Decreased release of IL-10 by monocytes from patients with lipoid nephrosis
1Second Department of Internal Medicine, Nihon University School of Medicine, Tokyo, Japan.
Insights
Interleukin-10 (IL-10) release is reduced in patients with lipoid nephrosis (LN), potentially increasing vascular permeability factor (VPF) activity. Measuring IL-10 may help monitor this kidney disease.
Area of Science:
- Immunology
- Nephrology
Background:
- Interleukin-10 (IL-10) is a cytokine that regulates immune responses, including B cell function and cytokine production.
- Monocytes are key immune cells involved in cytokine release and disease pathogenesis.
Purpose of the Study:
- To investigate the role of IL-10 in lipoid nephrosis (LN) by measuring its release from monocytes.
- To compare IL-10 levels in LN patients with healthy controls and patients with IgA nephropathy (IgAN).
Main Methods:
- Peripheral blood monocytes (PBM) were cultured from patients with LN, IgAN, and healthy controls.
- Spontaneous and lipopolysaccharide (LPS)-stimulated IL-10 release was measured in cell culture supernatants.
- IL-10 concentrations were correlated with vascular permeability factor (VPF) levels.
Main Results:
- IL-10 release was significantly decreased in LN patients compared to controls.
- IL-10 levels were lower in LN patients with nephrotic syndrome (NS) than in those without NS.
- No significant difference in IL-10 levels was observed in IgAN patients compared to controls.
- A negative correlation was found between IL-10 and VPF levels in LN patients.
Conclusions:
- Patients with active lipoid nephrosis exhibit a relative deficit in IL-10 release.
- Reduced IL-10 may contribute to increased VPF activity in active LN.
- IL-10 measurements could serve as a valuable biomarker for monitoring kidney disease activity in LN.
Abstract:
IL-10, a cross-regulatory cytokine produced by several cell types, including monocytes, is known to stimulate B cell growth and maturation and to inhibit cytokine production. In order to characterize further monocyte function in patients with lipoid nephrosis (LN), the release of IL-10 was measured in supernatants of cultured peripheral blood monocytes (PBM) that were obtained from LN patients and healthy controls. Spontaneous and lipopolysaccharide (LPS)-induced IL-10 release was decreased in patients with LN compared with those in normal controls and lower in LN patients with the nephrotic syndrome (NS) than in those without NS. In contrast, the values in IgA nephropathy (IgAN) patients with or without NS did not differ from normal subjects. There was a negative correlation between IL-10 concentration and the quantity of vascular permeability factor (VPF) released in LN patients. These imply that there is a relative deficit in IL-10 release in active LN, which suggests the possibility that inadequate release of IL-10 may lead to increased VPF activity in active LN patients and the measurements of IL-10 may be of value for monitoring kidney disease. The data provide the first detailed analysis of IL-10 in a group of patients with LN.