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Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine
09:25

Isolation and Characterization of Dendritic Cells and Macrophages from the Mouse Intestine

Published on: May 21, 2012

Enhanced interferon-gamma production and B7-2 expression in isolated intestinal mononuclear cells from patients with

M Noguchi1, N Hiwatashi, Z Liu

  • 1Third Department of Internal Medicine, Tohoku University School of Medicine, Sendai, Japan.

Journal of Gastroenterology
|November 1, 1995
PubMed

Insights

Interferon-gamma (IFN-gamma) levels and B7-2 expression are elevated in Crohn's disease intestinal tissues. This suggests a mechanism involving IFN-gamma and B7-2 in amplifying inflammatory responses in Crohn's disease.

Area of Science:

  • Immunology
  • Gastroenterology
  • Molecular Biology

Background:

  • Interferon-gamma (IFN-gamma) plays a role in inflammatory bowel disease (IBD), but its significance is debated.
  • IFN-gamma is known to upregulate B7-2 costimulatory molecules on monocytes.

Purpose of the Study:

  • To investigate IFN-gamma production and B7-2 expression in the intestinal mucosa and lamina propria mononuclear cells (LPMC) of patients with Crohn's disease (CD) and ulcerative colitis (UC).
  • To explore the role of IFN-gamma in modulating B7-2 expression on LPMC.

Main Methods:

  • Measurement of IFN-gamma levels in intestinal mucosa and isolated LPMC from CD, UC patients, and controls.
  • Flow cytometry analysis to assess B7-2 expression on LPMC.
  • Incubation of CD LPMC with endotoxin to evaluate B7-2 induction.

Main Results:

  • Mucosal IFN-gamma production was significantly higher in CD patients compared to controls, but not in UC patients.
  • Both IFN-gamma production and B7-2 expression were elevated in LPMC from CD patients relative to UC patients and controls.
  • Endotoxin stimulation further enhanced B7-2 expression on LPMC from CD patients.

Conclusions:

  • Elevated IFN-gamma production and B7-2 expression in the intestinal mucosa of CD patients suggest a specific role for this pathway in the disease.
  • The induction of B7-2 on LPMC by IFN-gamma may contribute to the amplification of T cell proliferation and lymphokine production in CD.

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