Interleukin 10 induced c-fos expression in human B cells by activation of divergent protein kinases

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H Bönig1, D Körholz, B Pafferath

  • 1Department of Pediatric Hematology and Oncology, Heinrich-Heine University Medical Center, Dusseldorf, Germany.

Immunological Investigations
|January 1, 1996
PubMed

Insights

Interleukin-10 (IL-10) significantly increases c-fos expression in human B cells within 30 minutes by activating tyrosine and serine/threonine kinases. This study elucidates IL-10 signal transduction pathways in B cell proliferation and differentiation.

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Interleukin-10 (IL-10) is a key regulator of human B cell growth and plasma cell differentiation.
  • The precise intracellular signaling mechanisms of IL-10 in B cells remain largely uncharacterized.
  • The protooncogene c-fos is implicated in B cell proliferation and differentiation.

Purpose of the Study:

  • To investigate the effect of IL-10 on the expression of the protooncogene c-fos in human B cells.
  • To elucidate the signal transduction pathways involved in IL-10-mediated c-fos expression.

Main Methods:

  • Human B cells were isolated and stimulated with IL-10.
  • c-fos messenger RNA (mRNA) expression was analyzed using a PCR-assisted mRNA assay.
  • The roles of tyrosine and serine/threonine kinases were assessed using specific inhibitors (genistein and H-7).

Main Results:

  • IL-10 induced a significant 2-4 fold increase in c-fos expression within 30 minutes, returning to basal levels after 2 hours.
  • The IL-10 effect was dose-dependent, with maximal stimulation at 100 ng/ml.
  • Inhibition of tyrosine kinases (genistein) and serine/threonine kinases (H-7) significantly blocked IL-10-induced c-fos expression.

Conclusions:

  • IL-10 stimulates c-fos expression in human B cells through the activation of both tyrosine and serine/threonine kinases.
  • This study provides the first report on IL-10-induced signal transduction in B cells, identifying key intracellular mechanisms.
  • Understanding these pathways is crucial for comprehending IL-10's role in B cell biology and related disorders.

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