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Updated: Aug 9, 2026
Generation of Human CD40-activated B cells
Published on: October 17, 2009
Effect of interleukin-10 on anti-CD40- and interleukin-4-induced immunoglobulin E production by human lymphocytes
Y Uejima1, K Takahashi, K Komoriya
1Pharmaceutical Discovery Research Laboratory, Teijin Institute for Biomedical Research, Tokyo, Japan.
Insights
Interleukin-10 (IL-10) enhances immunoglobulin (Ig) synthesis in human cells. This study shows IL-10 boosts IgE and IgG production by activating T lymphocytes and increasing IL-6.
Area of Science:
- Immunology
- Molecular Biology
Background:
- Interleukin-10 (IL-10) is a cytokine with diverse immunomodulatory functions.
- Understanding IL-10's role in immunoglobulin (Ig) synthesis is crucial for immune response research.
Purpose of the Study:
- To investigate the effect of IL-10 on in vitro IgE and IgG synthesis by human peripheral blood mononuclear cells (PBMC).
- To elucidate the mechanisms by which IL-10 influences Ig production.
Main Methods:
- Human PBMC were stimulated with anti-CD40 monoclonal antibody and IL-4.
- IL-10 levels, IgE and IgG production, cell proliferation, IL-6, and IFN-gamma were measured.
- Purified human tonsillar B cells were also used to assess IL-10 effects.
Main Results:
- Anti-CD40 and IL-4 stimulation increased IL-10, IgE, and IgG synthesis.
- Anti-IL-10 antibody suppressed IgE and IgG production without affecting PBMC proliferation.
- IL-10 enhanced IgE and IgG production, significantly increasing IL-6 but not IFN-gamma production.
- IL-10 enhanced B cell proliferation and IgG production in purified B cells, but not IgE production.
Conclusions:
- IL-10 accelerates IgE production in anti-CD40 and IL-4-stimulated PBMC.
- This acceleration appears to be mediated by enhanced IL-6 production through T lymphocyte activation.
- IL-10 plays a significant role in regulating Ig synthesis, particularly IgE, via T cell-dependent pathways.
Abstract:
We studied the effect of IL-10 on the in vitro synthesis of IgE and IgG by human peripheral blood mononuclear cells (PBMC) following stimulation with anti-CD40 monoclonal antibody and IL-4. Anti-CD40- and IL-4-stimulated PBMC showed an increase in IL-10 synthesis together with increases in IgE and IgG production. Addition of anti-IL-10 antibody to this system suppressed IgE as well as IgG production without affecting the proliferation of PBMC. Addition of IL-10 enhanced IgE and IgG production if PBMC were activated with anti-CD40 and IL-4. PBMC costimulated with anti-CD40, IL-4 and IL-10 showed a remarkable increase in IL-6 production, but had no effect on IFN-gamma production. Addition of IL-10 to purified human tonsillar B cells stimulated with anti-CD40 and IL-4 enhanced B cell proliferation and IgG production, but not IgE production. These results suggest that IL-10 accelerates IgE production by anti-CD40- and IL-4-stimulated PBMC by enhancing IL-6 production through activation of T lymphocytes.
