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Adhesion Frequency Assay for In Situ Kinetics Analysis of Cross-Junctional Molecular Interactions at the Cell-Cell Interface
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Identification of an active sequence within the first immunoglobulin domain of intercellular cell adhesion molecule-1

S E D'Souza1, V J Byers-Ward, E E Gardiner

  • 1Joseph J. Jacobs Center for Thrombosis and Vascular Biology, Department of Molecular Cardiology, The Cleveland Clinic Foundation, Cleveland, OH 44195, USA.

The Journal of Biological Chemistry
|September 27, 1996
PubMed

Insights

Monocytic cells use integrin alphaMbeta2 to bind fibrinogen (fg). This interaction enhances cell adhesion to endothelial cells via intercellular adhesion molecule-1 (ICAM-1), with a specific peptide region identified as key.

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Monocytic cells adhere to endothelial cells, a process crucial for immune responses.
  • Fibrinogen (fg) binding to monocytic cells via integrin alphaMbeta2 enhances this adhesion.
  • Intercellular adhesion molecule-1 (ICAM-1) plays a role in fg-mediated monocytic cell adhesion to endothelial cells.

Purpose of the Study:

  • To investigate the direct interaction between fibrinogen (fg) and intercellular adhesion molecule-1 (ICAM-1).
  • To identify specific regions within ICAM-1 and fg involved in their interaction.
  • To differentiate fg interactions with stimulated versus resting endothelial cells.

Main Methods:

  • Investigated direct binding of fg to intact and truncated forms of ICAM-1.
  • Utilized peptide mapping to identify the fg-binding site on ICAM-1 (ICAM-1-(8-21)).
  • Employed fg fragments and peptides (fg-gamma-117-133) to confirm binding specificity.
  • Assessed the role of ICAM-1-(8-21) and fg-gamma-117-133 in blocking cell adhesion.

Main Results:

  • A direct interaction between fg and ICAM-1 was demonstrated.
  • The peptide sequence ICAM-1-(8-21) within the first Ig domain of ICAM-1 specifically binds to fg fragments (X, D100, D80) but not fragment E.
  • The fg gamma-chain peptide (fg-gamma-117-133) blocked fg interaction with ICAM-1-(8-21).
  • ICAM-1-(8-21) peptide and anti-ICAM-1-(8-21) antibodies inhibited fg-mediated adhesion of ICAM-1-bearing cells.

Conclusions:

  • The ICAM-1-(8-21) region is a critical contact site for fg binding.
  • The fg gamma-chain peptide fg-gamma-117-133 is also involved in this interaction.
  • These specific molecular interactions mediate fg-ICAM-1-dependent monocytic cell adhesion.

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