Related Experiment Video

Updated: Aug 10, 2026

A Novel In vitro Model for Studying the Interactions Between Human Whole Blood and Endothelium
06:27

A Novel In vitro Model for Studying the Interactions Between Human Whole Blood and Endothelium

Published on: November 21, 2014

Bovine endothelial-like cells increase intercellular junctions under treatment with interferon-gamma. An in vitro

A Ricken1, C Rahner, L Landmann

  • 1Anatomisches Institut, Universität Basel, Switzerland.

Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft
|August 1, 1996
PubMed

Insights

Interferon-gamma (IFN-gamma) strengthens cell connections in bovine corpus luteum endothelial-like cells. This finding enhances our understanding of endothelial cell biology and intercellular junction regulation.

Area of Science:

  • Cell biology
  • Endothelial cell research
  • Corpus luteum function

Background:

  • Endothelial-like cells from the bovine corpus luteum can maintain cytokeratin (CK) expression in culture.
  • Understanding intercellular junction dynamics is crucial for vascular and tissue function.

Purpose of the Study:

  • To investigate the effect of interferon-gamma (IFN-gamma) on intercellular junctions in CK-positive endothelial-like cells.
  • To determine how IFN-gamma influences cell layer permeability.

Main Methods:

  • Long-term culture of bovine corpus luteum endothelial-like cells.
  • Immunofluorescence using monoclonal antibodies to detect intercellular junctions.
  • Electron microscopy for detailed ultrastructural analysis.
  • Horseradish peroxidase (HRP) assay to measure cell layer permeability.

Main Results:

  • IFN-gamma treatment increased the amount of intercellular junctions, including desmosomes, zonulae adhaerentes, and tight junctions.
  • Gap junctions were also observed but not quantified in terms of increase.
  • Cell layer permeability to HRP significantly decreased after IFN-gamma treatment.

Conclusions:

  • IFN-gamma reinforces intercellular junctions in CK-positive bovine endothelial-like cells.
  • The enhanced junctions lead to reduced cell layer permeability.
  • These findings contribute to understanding endothelial barrier function regulation.

More Related Videos

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice08:09

Vasodilation of Isolated Vessels and the Isolation of the Extracellular Matrix of Tight-skin Mice

Published on: March 24, 2017

Isolation of Primary Mouse Lung Endothelial Cells06:41

Isolation of Primary Mouse Lung Endothelial Cells

Published on: November 10, 2021

Related Concept Videos

TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
Inflammatory Response I: Vascular and Cellular01:30

Inflammatory Response I: Vascular and Cellular

The inflammatory response is the body's defense against infection, injury, or irritation from bacteria, trauma, toxins, or heat. Inflammation helps locate and destroy pathogens and remove damaged tissue elements to heal the body. During this initial phase, fluid, blood products, and nutrients migrate to the injured area, resulting in redness, heat, swelling, ache, and loss of function. Moreover, signs of systemic inflammation include fever, increased WBC count, malaise, anorexia, nausea,...