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Adhesion molecule expression by epidermal Langerhans cells and lymph node dendritic cells: a comparison

M Cumberbatch1, R J Dearman, I Kimber

  • 1Zeneca Central Toxicology Laboratory, Macclesfield, Cheshire, UK.

Archives of Dermatological Research
|November 1, 1996
PubMed

Insights

Epidermal Langerhans cells (LC) change adhesion molecule expression during migration to lymph nodes. E-cadherin decreases while ICAM-1 increases, impacting immune responses.

Area of Science:

  • Immunology
  • Dermatology
  • Cell Biology

Background:

  • Langerhans cells (LC) are crucial for skin immunity and antigen transport to lymph nodes.
  • LC undergo phenotypic changes during migration, affecting their immune functions.
  • Adhesion molecules on LC regulate interactions with tissues and T cells.

Purpose of the Study:

  • To investigate changes in E-cadherin, ICAM-1, and CD44 expression on migrating LC.
  • To understand the role of these adhesion molecules in LC functional maturation.

Main Methods:

  • Immunocytochemistry and flow cytometry were used to analyze adhesion molecule expression.
  • Expression levels were examined on LC in the skin and mature dendritic cells in lymph nodes.

Main Results:

  • LC migration was linked to a significant decrease in E-cadherin expression.
  • A parallel increase in intercellular adhesion molecule-1 (ICAM-1) expression was observed.
  • No detectable changes in CD44 expression were found on migrating LC.

Conclusions:

  • Altered E-cadherin and ICAM-1 expression are key events in LC migration and maturation.
  • These changes likely influence LC interactions and their capacity for antigen presentation.
  • The findings provide insights into the dynamic regulation of LC during cutaneous immune responses.

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