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The neuronal glycoprotein telencephalin is a cellular ligand for the CD11a/CD18 leukocyte integrin

L Tian1, Y Yoshihara, T Mizuno

  • 1Department of Biosciences, Division of Biochemistry, University of Helsinki, Finland.

Journal of Immunology (Baltimore, Md. : 1950)
|January 15, 1997
PubMed

Insights

Telencephalin (TLN), a novel central nervous system molecule, interacts with leukocyte integrins CD11a/CD18. This interaction, mediated by TLN's N-terminal domains, suggests a role in neuro-immune cell adhesion.

Area of Science:

  • Neuroscience
  • Immunology
  • Cell Biology

Background:

  • Leukocyte functions rely on beta2 integrins (CD11/CD18) binding to intercellular adhesion molecules (ICAMs).
  • Telencephalin (TLN), an Ig superfamily member, is expressed in the central nervous system and shares homology with known ICAM ligands.

Purpose of the Study:

  • To investigate the interaction between Telencephalin (TLN) and leukocyte integrins CD11a/CD18.
  • To identify the specific domains of TLN involved in this interaction.

Main Methods:

  • Adhesion assays using peripheral blood T cells, Jurkat T cells, and B lymphoblastoid cells with recombinant human TLN.
  • Inhibition studies using antibodies against CD11a/CD18.
  • Binding assays with TLN-transfected L cells and purified CD11a/CD18.
  • Testing binding of truncated TLN proteins (TLN(1-5) and TLN(1-9)) to CD11a/CD18.

Main Results:

  • Peripheral blood T cells, Jurkat T cells, and B lymphoblastoid cells demonstrated adhesion to recombinant TLN.
  • This adhesion was confirmed to be mediated by CD11a/CD18 and was inhibited by anti-CD11a/CD18 antibodies.
  • TLN-transfected cells also bound to purified CD11a/CD18, and TLN domains 1-5 were sufficient for binding.

Conclusions:

  • Telencephalin (TLN) is a novel neuronal cell adhesion molecule that interacts with CD11a/CD18.
  • The CD11a/CD18 recognition site on TLN is located within its N-terminal five Ig-like domains.
  • This interaction may play a significant role in integrin-mediated cell-cell interactions within the central nervous system.

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