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Updated: Aug 9, 2026
A Morphometric and Cellular Analysis Method for the Murine Mandibular Condyle
Published on: January 11, 2018
Cellular, biochemical and molecular characterization of the bovine temporomandibular joint disc
R Landesberg1, E Takeuchi, J E Puzas
1New York University, College of Dentistry, Department of Basic Sciences and Surgical Sciences, NY 10010, USA.
Insights
Bovine temporomandibular joint (TMJ) disc cells contain specific collagens and synthesize matrix components. These cells proliferate faster than bone or cartilage cells and respond to growth factors.
Area of Science:
- Biochemistry
- Cell Biology
- Orthopedics
Background:
- The temporomandibular joint (TMJ) disc is crucial for jaw function.
- Understanding its cellular and collagenous composition is vital for regenerative medicine.
Purpose of the Study:
- To analyze the cellular and collagenous components of the bovine TMJ disc.
- To compare the synthesis and proliferation rates of TMJ disc cells with other skeletal cells.
Main Methods:
- Isolation and analysis of cellular and collagenous components from bovine TMJ discs.
- Collagenase digestion, Western blot, and Northern blot analyses.
- In vitro culture of TMJ disc cells, osteoblasts, and chondrocytes.
Main Results:
- Type I, II, IX, and XII collagens were identified in the central TMJ disc regions.
- TMJ disc cells synthesized alkaline phosphatase, proteoglycans, and collagen.
- TMJ disc cells exhibited a lower basal synthesis rate but a higher proliferation rate compared to osteoblasts and chondrocytes.
- Transforming growth factor-beta significantly stimulated TMJ disc cell proliferation.
Conclusions:
- Bovine TMJ disc cells possess unique characteristics, including rapid proliferation and specific collagen expression.
- These findings provide insights into TMJ disc biology and potential therapeutic targets.
Abstract:
The cellular and collagenous components of the bovine temporomandibular joint (TMJ) disc have been isolated and analysed. In the central regions of the disc, significant amounts of type I, II, IX and XII collagen were found. The identity of these molecules was verified with collagenase digestions, Western blot analysis and Northern blot analysis (for type II collagen). Cells isolated from the TMJ disc synthesized alkaline phosphatase, proteoglycans and collagen in culture; however, the basal rate of synthesis for these molecules was lower than that for isolated osteoblasts, articular and growthplate chondrocytes. The TMJ disc cells proliferated more rapidly in culture than osteoblasts or chondrocytes. Transforming growth factor-beta stimulated proliferation by 250%, whereas prostaglandin E2 had no effect.
