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Lesional psoriatic T cells contain the capacity to induce a T cell activation molecule CDw60 on normal keratinocytes

L Skov1, L S Chan, D A Fox

  • 1Department of Dermatology, Gentofie Hospital, University of Copenhagen, Denmark.

The American Journal of Pathology
|February 1, 1997
PubMed

Insights

In psoriatic skin, T cells upregulate CDw60 on keratinocytes, partly via IL-13. This suggests a new mechanism for T cell involvement in psoriasis pathogenesis.

Area of Science:

  • Dermatology
  • Immunology
  • Cell Biology

Background:

  • Psoriatic skin exhibits altered keratinocyte characteristics.
  • CDw60, a ganglioside antigen, is expressed on keratinocytes.
  • The role of T cells in modulating CDw60 expression in psoriasis is unclear.

Purpose of the Study:

  • To investigate CDw60 expression in psoriatic skin keratinocytes.
  • To determine if T cell factors induce CDw60 overexpression on keratinocytes.
  • To identify specific T cell cytokines involved in CDw60 modulation.

Main Methods:

  • Analysis of CDw60 expression on basal and suprabasal keratinocytes in psoriatic skin.
  • Characterization of CDw60 antigen using monoclonal antibody UM4D4 and neuraminidase treatment.
  • Cloning of T cell lines from psoriatic lesions.
  • Incubation of normal keratinocytes with T cell clones and specific cytokines (IL-2, IL-3, IL-4, IL-6, IL-13, TGF-β, GM-CSF, IFN-γ).
  • Assessment of CDw60 expression modulation by T cell factors and cytokines.

Main Results:

  • CDw60 is expressed on basal and suprabasal keratinocytes in psoriatic skin.
  • CDw60 antigen is identified as the 9-O-acetylated disialosyl group on ganglioside GD3.
  • Activated T cell clones from psoriatic lesions up-regulated CDw60 expression on normal keratinocytes.
  • Interleukin-4 (IL-4) and Interleukin-13 (IL-13) strongly upregulated keratinocyte CDw60 expression.
  • Interferon-gamma (IFN-γ) downregulated keratinocyte CDw60 expression.
  • Anti-IL-13 partially neutralized the T cell supernatant-induced CDw60 upregulation.

Conclusions:

  • CDw60 expression on psoriatic epidermal keratinocytes is likely induced by intralesionally activated T cells.
  • IL-13 plays a significant role in the T-cell-mediated upregulation of CDw60.
  • This represents a novel mechanism of T cell participation in psoriasis pathogenesis.

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