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In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function
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Human immunodeficiency virus gp120 inhibits interleukin-12 secretion by human monocytes: an indirect

Y Taoufik1, O Lantz, C Wallon

  • 1Laboratoire Virus, Neurones et Immunité, Université Paris-Sud, Le Kremlin-Bicêtre, France.

Blood
|April 15, 1997
PubMed

Insights

Human immunodeficiency virus (HIV) gp120 protein impairs interleukin-10 (IL-10) and interleukin-12 (IL-12) production in monocytes and macrophages. This immune disregulation, driven by IL-10, may affect the immune response during HIV infection.

Area of Science:

  • Immunology
  • Virology
  • Cell Biology

Background:

  • Interleukin-12 (IL-12) is a cytokine produced by monocytes and macrophages with crucial immunomodulatory functions.
  • Understanding how viral infections impact cytokine production is vital for immune response modulation.

Purpose of the Study:

  • To investigate the effect of human immunodeficiency virus (HIV) gp120 on IL-12 production in monocytes and macrophages.
  • To elucidate the role of IL-10 in mediating potential gp120-induced immune dysregulation.

Main Methods:

  • Human monocytes and macrophages were preincubated with HIV-gp120.
  • Expression of IL-12 mRNA and protein levels was assessed following stimulation with Staphylococcus aureus strain cowan I (SAC).
  • Interleukin-10 (IL-10) synthesis was measured in response to HIV-gp120.

Main Results:

  • HIV-gp120 significantly decreased IL-12 mRNA subunit expression and protein production (p40 and p70) in monocytes and macrophages stimulated with SAC.
  • HIV-gp120 induced significant IL-10 synthesis in human monocyte cultures.
  • IL-10 was identified as the mediator inhibiting IL-12 mRNA accumulation and protein secretion.

Conclusions:

  • HIV-gp120 induces IL-10 production in monocytes and macrophages.
  • This IL-10 production leads to the suppression of IL-12 expression and secretion.
  • The resulting IL-10/IL-12 disregulation in uninfected cells may contribute to altered immune responses during HIV infection.

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