Related Experiment Video

Updated: Aug 9, 2026

Generation of Human CD40-activated B cells
13:27

Generation of Human CD40-activated B cells

Published on: October 17, 2009

Interleukin-13 responsiveness and interleukin-13 receptor expression in non-Hodgkin's lymphoma and reactive lymph

C Billard1, D Caput, N Vita

  • 1INSERM U. 448, Faculté de Médecine, Créteil, France.

European Cytokine Network
|March 1, 1997
PubMed

Insights

Interleukin-13 (IL-13) supports non-Hodgkin's lymphoma (NHL) B cell growth and survival. Responsiveness to IL-13 in NHL B cells is linked to IL-13 receptor expression and CD40 activation.

Area of Science:

  • Immunology
  • Oncology
  • Cell Biology

Background:

  • Interleukin-13 (IL-13) is a cytokine involved in immune responses.
  • B cells play a crucial role in both normal immune function and lymphomagenesis.
  • Understanding cytokine signaling in B cells is vital for developing targeted therapies.

Purpose of the Study:

  • To investigate the responsiveness of B cells from non-Hodgkin's lymphoma (NHL) patients to IL-13.
  • To determine the role of IL-13 receptors (IL-13R) and CD40 activation in IL-13 responsiveness.
  • To explore the potential impact of IL-13 on NHL B cell proliferation and survival.

Main Methods:

  • Proliferation assays were performed on lymph node B cells from NHL patients and healthy donors.
  • Binding experiments using radiolabeled IL-13 were conducted to assess IL-13 receptor expression.
  • CD40 activation and IL-10 treatment were used to modulate B cell responses.

Main Results:

  • A subset of NHL B cells exhibited IL-13 responsiveness without co-activation.
  • CD40 activation induced IL-13 responsiveness in previously unresponsive NHL and reactive B cells.
  • IL-13R expression correlated with IL-13 responsiveness, and CD40 activation upregulated IL-13R.
  • IL-13 enhanced the viability of cultured NHL cells, but not non-malignant cells.

Conclusions:

  • IL-13 may promote growth and survival advantages for NHL B cells.
  • IL-13 signaling, modulated by IL-13R and CD40, represents a potential therapeutic target in NHL.

More Related Videos

Murine Model of CD40-activation of B cells12:24

Murine Model of CD40-activation of B cells

Published on: March 6, 2010

A BW Reporter System for Studying Receptor-Ligand Interactions06:05

A BW Reporter System for Studying Receptor-Ligand Interactions

Published on: January 7, 2019

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...