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Published on: October 21, 2016
Instantaneous cloth-based enzyme immunoassay for the semi-quantitative visual determination of antibodies
1Department of Biology and Institute of Biochemistry, Carleton University Ottawa, Ontario, Canada.
Insights
A novel blotting assay uses an antigen-coated cloth to detect antibodies in samples. This method allows for rapid field serodiagnosis of diseases in humans and animals.
Area of Science:
- Immunological assays
- Biochemical detection methods
Background:
- Accurate serodiagnosis is crucial for disease control in both human and animal populations.
- Field-deployable diagnostic tools are needed for rapid disease identification and management.
Purpose of the Study:
- To develop a rapid and simple assay for antibody detection.
- To enable field serodiagnosis of diseases.
Main Methods:
- An antigen-coated cloth was used as the solid phase for antibody capture.
- The assay involved sequential blotting with antibody sample, washing, anti-antibody-peroxidase conjugate, washing, and peroxidase substrate.
- Detection of the colored product was achieved visually or colorimetrically.
Main Results:
- The intensity of the color produced was directly correlated with the antibody concentration in the sample.
- The assay demonstrated potential for rapid antibody quantification.
Conclusions:
- This blotting assay provides a quick and effective method for antibody detection.
- The assay is suitable for field serodiagnosis, aiding in the management of infectious diseases.
Abstract:
An antigen-coated cloth segment was placed onto a blotting pad. The cloth was blotted with one drop of an antibody sample, immediately washed with five drops of a washing solution, exposed to one drop of an anti-antibody-peroxidase conjugate, washed, and finally exposed to a peroxidase substrate. The colored peroxidase product was determined either visually or colorimetrically with the color intensity being correlated to the antibody concentration. This rapid assay would facilitate field serodiagnosis of human and animal diseases.
