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Human endometrial leukemia inhibitor factor (LIF) secretion and its relationship to sonographic endometrial
1Department of OB/GYN, Hospital Antoine Beclere, Clamart, France.
Insights
Leukemia inhibitory factor (LIF) is crucial for human reproduction. High endometrial LIF levels negatively impact endometrial growth and receptivity, potentially hindering embryo implantation during the proliferative phase.
Area of Science:
- Reproductive Endocrinology
- Cellular Biology
- Gynecology
Background:
- Leukemia inhibitory factor (LIF) plays a role in human reproduction.
- Its function during the proliferative phase of the menstrual cycle and its effect on endometrial growth are not well understood.
Purpose of the Study:
- To investigate the relationship between endometrial LIF secretion and endometrial growth.
- To evaluate the correlation between endometrial LIF concentration and ultrasound-assessed endometrial characteristics.
Main Methods:
- Prospective, blinded study involving 24 women undergoing IVF.
- Endometrial biopsy and ultrasound measurements of endometrial thickness and pattern were performed.
- Endometrial LIF concentration was quantified using ELISA on cultured endometrial explants.
Main Results:
- Endometrial LIF production showed a significant negative correlation with endometrial thickness (P < 0.05).
- LIF production was elevated 4-fold when endometrial thickness was less than 5 mm.
- A strong correlation was observed between LIF production and sonographic endometrial pattern, with higher LIF levels in unfavorable endometria.
Conclusions:
- A dynamic relationship exists between endometrial sonographic appearance and LIF secretion during the proliferative phase.
- Negative correlation between LIF production, endometrial thickness, and pattern suggests an inhibitory effect on endometrial growth.
- Overexpression of LIF may lead to an unfavorable uterine environment for endometrial development, impacting reproductive success.
Problem:
LIF is believed to be involved in human reproduction. Because little is known about the function of this cytokine in proliferative phase of cycle and because LIF is found to regulate the cellular growth we evaluated the possible relationship between endometrial LIF secretion and endometrial growth.
Method:
The present is prospective, blinded study with clinical and immunobiochemical correlation between endometrial LIF concentration and endometrial ultrasound pattern. Twenty-four patients who were candidates for IVF and oocyte donation are included in this study. At day 10 of their cycle the endometrial biopsy was performed, followed by vaginal sonographic measurements of endometrial thickness and pattern. Endometrial LIF concentration was measured by ELISA in supernatants taken from cultured explants.
Results:
Endometrial LIF production significantly negatively correlated with endometrial thicknesses (P < 0.05). There was a 4-fold elevation in LIF production when the endometrial thickness was below 5 mm. Strong correlation was found also between endometrial LIF production and sonographic endometrial pattern (P < 0.05). The most significant amount of LIF was found in nonfavorable endometria.
Conclusion:
In the proliferative phase of cycle there is a dynamic relationship between endometrial sonographic appearance and local LIF secretion. Specifically LIF production is negatively correlated with endometrial thickness and pattern. The low amount of cytokine is a normal uteral environment for endometrial development, whereas deregulation of cytokine production toward its overexpression may lead to a strong inhibitory effect on endometrial growth. This finding might have an important clinical implication.
