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Comparative effects of bovine cytokines on cattle and bison peripheral blood mononuclear cell proliferation
M G Stevens1, S C Olsen, N F Cheville
1National Animal Disease Center, USDA, Ames, Iowa, 50010, USA.
Insights
Bovine interleukin-2 (BoIL-2) and interleukin-1 beta (BoIL-1 beta) stimulate cattle and bison peripheral blood mononuclear cell (PBMC) proliferation. Granulocyte-macrophage colony-stimulating factor (BoGM-CSF) did not significantly affect PBMC proliferation in this study.
Area of Science:
- Immunology
- Veterinary Immunology
- Cell Biology
Background:
- Peripheral blood mononuclear cells (PBMC) are crucial for immune responses in cattle and bison.
- Understanding cytokine effects on PBMC proliferation is vital for animal health research.
Purpose of the Study:
- To investigate the effects of specific bovine cytokines on the proliferation of cattle and bison PBMC.
- To determine if bovine interleukin 1 beta (BoIL-1 beta), interleukin 2 (BoIL-2), and granulocyte-macrophage colony-stimulating factor (BoGM-CSF) can induce or enhance PBMC proliferation.
Main Methods:
- PBMC from cattle and bison were isolated and cultured.
- Cells were stimulated with varying concentrations of BoIL-1 beta, BoIL-2, or BoGM-CSF, alone or in combination with mitogens like Con A, PHA, PWM, and LPS.
- PBMC proliferation was measured after 48 hours of incubation.
Main Results:
- BoIL-2 alone induced significant PBMC proliferation in both species.
- BoIL-1 beta and BoIL-2 enhanced mitogen-induced PBMC proliferation.
- BoGM-CSF did not induce or enhance PBMC proliferation under the tested conditions.
Conclusions:
- BoIL-1 beta and BoIL-2 play a role in stimulating cattle and bison PBMC proliferation.
- BoGM-CSF does not appear to be a significant stimulator of PBMC proliferation in these species.
- These findings contribute to understanding bovine and bison immune cell function.
Abstract:
Proliferation of peripheral blood mononuclear cells (PBMC) from cattle and bison was measured following stimulation of PBMC with bovine cytokines. Bovine interleukin 1 beta (BoIL-1 beta), interleukin 2 (BoIL-2) or granulocyte-macrophage colony-stimulating factor (BoGM-CSF) at 0.1-100 U/ml were incubated for 48 h with PBMC alone or with PBMC and various mitogens. These included concanavalin A (Con A), phytohemagglutinin (PHA), pokeweed mitogen (PWM) or Escherichia coli 055:B5 lipopolysaccharide (LPS) at 10-0.1 micrograms/ml. BoIL-2 alone, but not BoIL-1 beta and BoGM-CSF alone, induced proliferation of cattle and bison PBMC in the absence of mitogens. In addition, BoIL-1 beta and BoIL-2, but not BoGM-CSF, enhanced proliferation of cattle and bison PBMC induced by mitogens. These results indicate that BoIL-1 beta and BoIL-2 stimulate cattle and bison PBMC proliferation in a similar manner, whereas BoGM-CSF does not appear capable of stimulating either cattle or bison PBMC proliferation.
