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Subcellular distribution of 220 kDa antigen in the intercellular spaces of normal human epidermis

H Suzuki1, M Horii, R Miyamoto

  • 1Department of Dermatology, Surugadai Nihon University Hospital, Tokyo, Japan.

Archives of Dermatological Research
|May 1, 1997
PubMed

Insights

Researchers identified a 220 kDa epidermal protein involved in skin barrier function. This protein originates in the Golgi apparatus and is secreted into intercellular spaces, playing a role in epidermal differentiation.

Area of Science:

  • Dermatology
  • Cell Biology
  • Biochemistry

Background:

  • The epidermis forms a crucial barrier against environmental factors.
  • Understanding epidermal proteins is key to deciphering skin barrier function and differentiation.

Purpose of the Study:

  • To characterize a novel 220 kDa epidermal protein identified using a monoclonal antibody (7C1).
  • To investigate the subcellular localization and potential function of this protein in human epidermis.

Main Methods:

  • Monoclonal antibody generation (7C1) against pig snout skin extract.
  • Immunoblotting to identify the antigen as a 220 kDa protein.
  • Immunoelectron microscopy to determine subcellular localization in normal human skin.

Main Results:

  • The 220 kDa protein is primarily located in the intercellular spaces of living epidermal layers.
  • Subcellular localization includes Golgi apparatus, lamellar granules, intercellular spaces, and vesicles.
  • Protein distribution altered in certain keratinizing disorders.

Conclusions:

  • The 220 kDa protein is synthesized in the Golgi apparatus and secreted into intercellular spaces via lamellar granules or vesicles.
  • This protein is likely involved in maintaining the epidermal permeability barrier.
  • The protein may also play a role in keratinizing differentiation of the epidermis.

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