Related Experiment Video

Updated: Aug 16, 2026

Murine Model of CD40-activation of B cells
12:24

Murine Model of CD40-activation of B cells

Published on: March 6, 2010

IL-10 production and CD40L expression in patients with common variable immunodeficiency

A Oliva1, E Scala, I Quinti

  • 1Department of Allergy and Clinical Immunology, La Sapienza University of Rome, Italy.

Scandinavian Journal of Immunology
|July 1, 1997
PubMed

Insights

Common variable immunodeficiency (CVI) patients show normal interleukin-10 (IL-10) production, but CD40 ligand (CD40L) expression may be functionally defective in some cases, impacting IgG production.

Area of Science:

  • Immunology
  • Clinical Medicine
  • Molecular Biology

Background:

  • Common variable immunodeficiency (CVI) is characterized by impaired antibody production.
  • The roles of CD40 ligand (CD40L) and interleukin-10 (IL-10) in CVI pathogenesis are not fully understood.

Purpose of the Study:

  • To investigate CD40L expression and IL-10 production in CVI patients.
  • To determine the potential contribution of these factors to impaired IgG production in CVI.

Main Methods:

  • Flow cytometry was used to measure CD40L expression on peripheral blood mononuclear cells (PBMCs) from CVI patients and controls.
  • IL-10 production was assessed after in vitro stimulation of PBMCs.
  • In vitro IgG production was induced using anti-CD40 and cytokine stimulation.

Main Results:

  • CD40L expression was comparable to controls in most CVI patients, but functional defects were observed in some.
  • IL-10 production was similar in CVI patients and controls.
  • In vitro stimulation induced significant IgG production in only one of 13 CVI patients tested.

Conclusions:

  • Altered IL-10 production is unlikely to be a major factor in impaired IgG production in most CVI patients.
  • While CD40L is often normally expressed, functional defects may contribute to CVI in a subset of patients.
  • Further research is needed to elucidate the precise mechanisms underlying CVI pathogenesis.

More Related Videos

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses10:13

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

Whole Blood Assay with Dual Co-Stimulation for Antigen-Specific Analysis of Host Immunity to Fungal and Viral Pathogens06:03

Whole Blood Assay with Dual Co-Stimulation for Antigen-Specific Analysis of Host Immunity to Fungal and Viral Pathogens

Published on: September 20, 2024

Related Concept Videos

Lymphoid Cells and Tissues01:18

Lymphoid Cells and Tissues

Lymphoid cells and tissues are integral to the immune system, which is crucial in maintaining our body's defense against harmful pathogens. They form the building blocks of lymphoid organs, which include the spleen, thymus, and lymph nodes.
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
Immunodeficiency Diseases01:25

Immunodeficiency Diseases

Immunodeficiency disorders are conditions in which the immune system's ability to fight infectious disease and cancer is compromised or entirely absent. The immune system comprises a complex network of cells, tissues, and organs that work together to protect the body from potentially harmful invaders. When this system is deficient or not functioning properly, it leaves the body susceptible to infections, diseases, or other complications.
There are three main causes of immunodeficiency disorders...