CD84 leukocyte antigen is a new member of the Ig superfamily

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M A de la Fuente1, P Pizcueta, M Nadal

  • 1Fundació Clínic per a la Recerca Biomèdica, the University of Barcelona, Spain.

Blood
|October 6, 1997
PubMed

Insights

A novel cell-surface glycoprotein, HLy9-beta (now CD84), was identified in human B-cells. This immunoglobulin superfamily member is expressed on leukocytes and may be crucial for immune cell activation.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • The immunoglobulin (Ig) superfamily plays critical roles in cell recognition and adhesion.
  • Novel cell-surface proteins are continually being discovered, expanding our understanding of cellular interactions.

Purpose of the Study:

  • To identify and characterize a novel cell-surface glycoprotein from a human B-cell line.
  • To determine the structural and functional properties of this new protein and its relationship to known Ig superfamily members.

Main Methods:

  • cDNA library screening and sequencing from a human B-cell line (Raji).
  • Northern blot analysis for gene expression profiling in hematopoietic tissues.
  • Chromosome localization studies to map the gene.
  • Transfection of cells with cloned cDNA and analysis using monoclonal antibodies (MoAbs).

Main Results:

  • A full-length cDNA sequence for a novel glycoprotein, HLy9-beta, was identified.
  • HLy9-beta possesses structural homology to known Ig superfamily members and is predominantly expressed in hematopoietic tissues.
  • The gene for HLy9-beta was mapped to chromosome 1q24.
  • CD84 MoAbs reacted with transfected cells, identifying HLy9-beta as CD84, a highly glycosylated glycoprotein (64-82 kDa) expressed on lymphocytes and monocytes.

Conclusions:

  • HLy9-beta is a newly identified member of the immunoglobulin superfamily, now recognized as CD84.
  • CD84 is a cell-surface glycoprotein predominantly expressed on lymphocytes and monocytes.
  • CD84 may play a significant role in leukocyte activation.

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