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Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell Blocks
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Cyclin D1 protein in multiple myeloma and plasmacytoma: an immunohistochemical study using fixed, paraffin-embedded

M A Vasef1, L J Medeiros, L S Yospur

  • 1Division of Pathology, City of Hope National Medical Center, Duarte, CA 91010, USA.

Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc
|October 6, 1997
PubMed

Insights

Cyclin D1 protein is expressed in about 26% of plasma cell neoplasms, including multiple myeloma. This expression correlates with tumor differentiation and stage, suggesting mechanisms beyond the t(11;14) translocation.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pathology

Background:

  • Plasma cell neoplasms are a group of hematologic malignancies.
  • Cyclin D1 is a cell cycle regulatory protein.
  • The t(11;14) translocation is a known genetic abnormality in some plasma cell neoplasms.

Purpose of the Study:

  • To investigate the expression of cyclin D1 protein in various plasma cell neoplasms.
  • To determine the correlation between cyclin D1 expression and clinicopathologic features.
  • To explore the relationship between cyclin D1 expression and the t(11;14) translocation.

Main Methods:

  • Immunohistochemistry was used to detect cyclin D1 protein in 69 plasma cell neoplasms (54 multiple myelomas, 3 plasma cell leukemias, 12 plasmacytomas).
  • Tissue sections were routinely fixed and paraffin-embedded.
  • Polymerase chain reaction (PCR) was used to analyze for the t(11;14) translocation in a subset of cases.

Main Results:

  • Cyclin D1 protein was expressed in 18 (26%) of 69 cases.
  • Expression was observed in 16 (30%) of multiple myelomas and 2 (17%) of plasmacytomas.
  • Cyclin D1 expression correlated with intermediate/immature cytologic features and histologic Stage III in multiple myeloma.
  • Only one multiple myeloma case harbored the t(11;14) translocation and expressed cyclin D1.

Conclusions:

  • Cyclin D1 protein expression is found in approximately one-quarter of plasma cell neoplasms.
  • Cyclin D1 expression is associated with specific cytologic and histologic characteristics of multiple myeloma.
  • The frequent cyclin D1 expression, relative to the incidence of t(11;14), suggests alternative regulatory mechanisms are involved.

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