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Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes
12:50

Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes

Published on: August 9, 2011

Recombinant Listeria monocytogenes as a live vaccine vehicle and a probe for studying cell-mediated immunity

E R Jensen1, H Shen, F O Wettstein

  • 1Department of Microbiology and Immunology, University of California at Los Angeles School of Medicine, USA.

Immunological Reviews
|August 1, 1997
PubMed

Insights

Listeria monocytogenes can be engineered as a vaccine vehicle to induce long-term immunity. Recombinant Listeria strains expressing foreign antigens successfully generated protective CD8+ T-cell responses in animal models.

Area of Science:

  • Immunology
  • Microbiology
  • Vaccinology

Background:

  • Listeria monocytogenes' cytosolic entry facilitates antigen presentation via MHC class I.
  • L. monocytogenes is a promising live vaccine vehicle for heterologous antigens.
  • Induction of immunological memory and CD8+ T-cell responses are key vaccine goals.

Purpose of the Study:

  • To establish a genetic system for regulated antigen expression and secretion by recombinant Listeria.
  • To evaluate the capacity of these recombinant strains to induce long-term immunological memory and protective immunity.
  • To assess the efficacy of L. monocytogenes-based vaccines in viral and tumor models.

Main Methods:

  • Developed a site-specific genetic integration system for antigen expression cassettes in Listeria.
  • Utilized lymphocytic choriomeningitis virus (LCMV) model in mice to assess CD8+ T-cell induction and protection.
  • Employed a cottontail rabbit papillomavirus model in rabbits to evaluate antitumor immunity.

Main Results:

  • Recombinant Listeria strains expressing LCMV antigens successfully induced LCMV-specific CD8+ T cells in mice.
  • Vaccinated mice demonstrated protection against LCMV challenge.
  • Recombinant Listeria stimulated protective antitumor immunity in a rabbit papillomavirus model.

Conclusions:

  • Recombinant L. monocytogenes vaccines demonstrate protective efficacy against viral challenge and tumors.
  • The established experimental system enables systematic analysis of cytotoxic T-cell induction by intracellular bacteria.
  • L. monocytogenes serves as an effective platform for developing vaccines against various pathogens and cancers.

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