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Capillary electrophoresis based immunoassays: a critical review

D Schmalzing1, W Nashabeh

  • 1Whitehead Institute, Cambridge, MA, USA. schmalzing@wi.mit.edu

Electrophoresis
|February 10, 1998
PubMed

Insights

Capillary electrophoresis (CE) offers significant potential for immunoassay analysis (IA). This review explores CE applications in research and diagnostics, including microfluidic devices, for enhanced immunoassays.

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Immunology

Background:

  • Immunoassays (IA) are crucial for research and clinical diagnostics, requiring sensitive and specific detection methods.
  • Combining capillary electrophoresis (CE) with IA offers a promising avenue for improving assay performance and efficiency.
  • Current research focuses on integrating CE principles to meet the evolving demands of modern immunoassay analysis.

Purpose of the Study:

  • To review and evaluate the uses and potentials of capillary electrophoresis (CE) in immunoassay analysis (IA).
  • To discuss the fundamental requirements and applications of immunoassays in research and clinical diagnostics.
  • To explore the integration of CE with IA, including microfabricated devices, and assess the technology's current status.

Main Methods:

  • Review of existing literature on capillary electrophoresis (CE) applications in immunoassay analysis (IA).
  • Discussion of different modes of CE utilization within IA.
  • Investigation of immunoassays performed on microfabricated devices as an alternative to capillary-based CE.

Main Results:

  • Capillary electrophoresis (CE) demonstrates diverse applications and significant potential for enhancing immunoassay analysis (IA).
  • Various CE modes are applicable to IA, offering tailored solutions for different analytical needs.
  • Microfabricated devices present a viable alternative to conventional capillary-based CE for immunoassays.

Conclusions:

  • Capillary electrophoresis (CE) is a valuable technology for advancing immunoassay analysis (IA) in both research and clinical settings.
  • The integration of CE, particularly with microfluidic platforms, holds promise for more efficient and sensitive immunoassays.
  • Further critical assessment is needed to fully realize the merits and potential of CE in immunoassay development.

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