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Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis
09:01

Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis

Published on: November 17, 2020

Immunological differentiation between tickborne encephalitis with and without concomitant neuroborreliosis

J Tomazic1, A Ihan, F Strle

  • 1Department of Infectious Diseases, University Medical Centre Ljubljana, Slovenia.

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
|March 12, 1998
PubMed

Insights

Flow cytometry analysis of cerebrospinal fluid revealed distinct lymphocyte subset differences between tick-borne encephalitis (TBE) and TBE with neuroborreliosis. These immune cell profiles can help diagnose co-occurring neuroborreliosis in TBE patients.

Area of Science:

  • Neuroimmunology
  • Infectious Diseases

Background:

  • Tick-borne encephalitis (TBE) and neuroborreliosis are serious tick-borne infections affecting the central nervous system.
  • Differentiating these conditions, especially when co-occurring, is crucial for appropriate patient management.

Purpose of the Study:

  • To analyze cerebrospinal fluid (CSF) lymphocyte subsets in patients with TBE versus those with TBE and concomitant neuroborreliosis (double infection).
  • To identify specific immune cell markers that can help diagnose double infections.

Main Methods:

  • Flow cytometry was used to analyze lymphocyte subsets in the CSF of patients.
  • Statistical analysis, including logistic regression, was performed to compare groups and identify predictive variables.

Main Results:

  • Patients with TBE showed higher percentages of CD4+DR+ and CD25+ T cells.
  • Patients with double infection exhibited higher percentages of CD19+ cells, CD8+DR- T cells, and CD3+CD71+ T cells.
  • CD19+ cells, CD3+CD25+ T cells, and CD3+CD71+ T cells were significant in a logistic regression model for predicting double infection.

Conclusions:

  • Distinct differences in CSF lymphocyte subsets exist between TBE and TBE with neuroborreliosis.
  • Flow cytometric analysis of CSF lymphocytes can aid in diagnosing concomitant neuroborreliosis in TBE patients.

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