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Immunogenic PDGF-AB dimers in crude leukocyte interferon batches
Insights
Sendai virus infection of human leukocytes produces interferons and platelet-derived growth factor AB (PDGF-AB). Acidification to inactivate the virus altered PDGF-AB levels, favoring dimer formation.
Area of Science:
- Immunology
- Virology
- Molecular Biology
Background:
- Human peripheral blood leukocytes (hPBL) infected with Sendai virus produce human leukocyte interferons (IFN-alpha n3) and other cytokines.
- Virus-infected hPBL supernatants contain significant amounts of platelet-derived growth factor AB (PDGF-AB).
Purpose of the Study:
- To quantify immunogenic PDGF-AB in interferon batches.
- To assess the impact of Sendai virus inactivation by acidification on PDGF-AB content and form.
Main Methods:
- Quantitative enzyme-linked immunosorbent assay (ELISA) was used to determine PDGF-AB concentrations.
- Interferon batches were analyzed before and after Sendai virus inactivation via acidification (pH 2 for 5 days).
Main Results:
- Immunogenic PDGF-AB was detected in all interferon batches, both before and after acidification.
- The virus inactivation process significantly altered the content of immunogenic PDGF-AB.
- Acidification favored the appearance of PDGF-AB dimers in most samples.
Conclusions:
- Sendai virus inactivation by acidification influences the levels and forms of PDGF-AB present in interferon preparations.
- This finding is relevant for understanding cytokine production and purification processes.
Abstract:
The infection of human peripheral blood leukocytes (hPBL) with Sendai virus in vitro, induces the synthesis of human leukocyte interferons (IFN-alpha n3) as well as other cytokines. The authors discovered that supernatants of virus-infected hPBL also contained significant amounts of platelet-derived growth factor AB (PDGF-AB). The concentrations of immunogenic PDGF-AB in the interferon batches were determined by quantitative enzyme-linked immunosorbent assay (ELISA) before and after the inactivation of Sendai virus by acidification (pH 2) for 5 days. Immunogenic PDGF-AB molecules were detected in all interferon batches before and after acidification. The virus inactivation process caused a significant change in the content of immunogenic PDGF-AB in the interferon samples. Inactivation of the Sendai virus by acidification favoured the appearance of PDGF-AB dimers in the majority of samples.