Related Experiment Video
Updated: Aug 8, 2026
Automated Microfluidic Blood Lysis Protocol for Enrichment of Circulating Nucleated Cells
Published on: December 31, 2009
[Cytometric analysis of lymphocyte phenotype in cord and newborn blood]
M Banasik1, K Zeman, A Malinowski
1Pracowni Cytometrii Przepływowej Zakładu Immunologii Klinicznej CZMP.
Insights
This study establishes crucial reference values for infant lymphocyte subpopulations by comparing cord blood and early infant samples. These findings aid in diagnosing immunological disorders and intrauterine infections in newborns.
Area of Science:
- Immunology
- Pediatrics
- Flow Cytometry
Background:
- Investigating the immune system in early life is vital for diagnosing primary immunodeficiencies.
- Prenatal infections can impact infant immune system development.
- Flow cytometry is essential for detailed immune system analysis.
Purpose of the Study:
- To establish age-matched reference values for lymphocyte subpopulations in healthy infants.
- To compare lymphocyte subset profiles between cord blood and early postnatal samples.
- To provide data for diagnosing immunological disorders and intrauterine infections.
Main Methods:
- Flow cytometric immunophenotyping was used to analyze lymphocyte subsets.
- Paired blood samples from healthy infants (cord blood and day 3) were analyzed.
- Data were compared to those of older children (7-17 years).
Main Results:
- Significant age-related differences were observed in various lymphocyte subpopulations.
- Established reference values for early life lymphocyte immunophenotyping.
- Demonstrated distinct immune profiles at birth versus early infancy.
Conclusions:
- The study provides essential age-matched reference data for infant lymphocyte immunophenotyping.
- These reference values are critical for accurate diagnosis of immunological disorders.
- The findings support enhanced diagnostic and prognostic capabilities for neonatal care.
Abstract:
At birth and in early childhood, phenotypic and functional investigation of the immune system is often necessary to identify primary immunological disorders and may provide additional diagnostic and prognostic information in certain intrauterine infections. We compared, using of flow cytometric immunophenotyping, a broad panel of lymphocyte subsets in paired samples obtained from healthy infants at birth (cord blood) and at 3 days of age. Results were compared to those of normal children (7 to 17 years) evaluated using the same methods. Significant age-related differences were demonstrated in lymphocyte subpopulations. Our data can be used as age-matched reference values for blood lymphocyte immunophenotyping.
