Related Experiment Video
Updated: Aug 13, 2026
New Tools to Expand Regulatory T Cells from HIV-1-infected Individuals
Published on: May 30, 2013
A CD4+ proliferation of large granular lymphocytes expresses the protease activated receptor-1
Insights
Large granular lymphocytes (LGLs) expressing PAR-1 were found in CD4+ CD57+ monoclonal expansions. This suggests PAR-1 involvement in rare LGL proliferations.
Area of Science:
- Immunology
- Cell Biology
- Molecular Biology
Background:
- Protease-activated receptors (PARs) are a family of G protein-coupled receptors activated by proteases.
- PAR-1, the first identified member, is known as the platelet-type thrombin receptor.
- Previous research indicated PAR-1 expression on CD8+ large granular lymphocytes (LGLs) in certain proliferative conditions.
Observation:
- This study investigated PAR-1 expression in a rare case of CD4+ CD57+ monoclonal LGL expansion.
- Three-color immunofluorescence was employed to detect PAR-1 and other cell markers.
Findings:
- Large granular lymphocytes (LGLs) from the patient with CD4+ CD57+ monoclonal expansion expressed PAR-1.
- Northern blot analysis confirmed high levels of PAR-1 mRNA in these LGLs, indicating active gene expression.
- The findings extend previous observations of PAR-1 expression in LGLs to a different immunophenotype.
Implications:
- The presence of PAR-1 on CD4+ CD57+ LGLs suggests a potential role for thrombin signaling in this rare lymphoproliferative disorder.
- Understanding PAR-1's function in these cells could offer new therapeutic targets.
- Further research is warranted to elucidate the specific mechanisms and clinical significance of PAR-1 in CD4+ CD57+ LGL expansions.
Abstract:
The platelet-type thrombin receptor was the first member to be identified in a family of protease activated receptors (PARs) and has been designated PAR-1. We recently reported that the large granular lymphocytes (LGLs) in patients with proliferations of CD8+ cells co-expressed PAR-1 and the expression of PAR-1 correlated with the expression of CD57. Here we show, by three-colour immunofluorescence, that the LGLs from a patient with a rare CD4+ CD57+ monoclonal expansion also expressed PAR-1. Northern blot analysis confirmed the presence of high levels of mRNA for PAR-1 in these LGLs.
More Related Videos
08:11Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.
Published on: September 1, 2015
06:56A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4
Published on: March 10, 2018
