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Updated: Aug 14, 2026

Isolation and Activation of Murine Lymphocytes
08:08

Isolation and Activation of Murine Lymphocytes

Published on: October 30, 2016

Interleukin-12-dependent activation of human lymphocyte subsets

O J Cordero1, F J Salgado, J E Viñuela

  • 1Department of Biochemistry and Molecular Biology, University of Santiago de Compostela, Spain. bnojcord@usc.es

Immunology Letters
|May 15, 1998
PubMed

Insights

Interleukin-12 (IL-12) enhances CD8 T cell proliferation and CD26 expression in activated T cells. This finding suggests a novel pathway for T-helper 1 (Th1) immune responses with potential clinical applications.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • Interleukin-12 (IL-12) is known to influence T-helper 1 (Th1) immune responses.
  • Previous research indicated IL-12 increases CD26/dipeptidyl peptidase IV (DPPIV) expression and function, suggesting a new cellular pathway.

Purpose of the Study:

  • To identify specific T cell subsets that respond to IL-12-induced CD26 upregulation.
  • To investigate the effects of IL-12 on T cell proliferation and CD26 expression under specific culture conditions.

Main Methods:

  • Utilized dual fluorescence analysis to examine CD26 expression on activated T cells.
  • Cultured T cells with phytohemagglutinin (PHA) and IL-12 to assess proliferation and marker expression.

Main Results:

  • IL-12 preferentially enhanced CD8 T cell proliferation, contrasting with some previous findings.
  • IL-12-dependent CD26 expression was observed on both CD4 and CD8 activated T cells.
  • While the percentage of CD45RO+ cells remained unaffected, the density of CD45RO antigen expression was reduced.

Conclusions:

  • IL-12 modulates T cell subsets, notably enhancing CD8 T cell proliferation and influencing CD26 and CD45RO expression.
  • These immunomodulatory effects of IL-12 may have implications for Th1-like immune responses.
  • The findings suggest potential clinical applications for IL-12 in immune modulation.

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